Identification of TRP-2 as a human tumor antigen recognized by cytotoxic T lymphocytes

Rong Fu Wang, Ettore Appella, Yutaka Kawakami, Xiaoqiang Kang, Steven A. Rosenberg

Research output: Contribution to journalArticle

260 Citations (Scopus)

Abstract

The infusion of TIL586 along with interleukin-2 into the autologous patient with metastatic melanoma resulted in the objective regression of tumor. A gene encoding a tumor antigen recognized by TIL586 was previously isolated and shown to encode gp75 or TRP-1. Here we report that TRP-2 was identified as a second tumor antigen recognized by a HLA-A31-restricted CTL clone derived from the TIL586 cell line. The peptide LLPGGRPYR epitope was subsequently identified from the coding region of TRP-2 based on studies of the recognition of truncated TRP-2 cDNAs and the HLA-A31 binding motif. This epitope peptide was capable of sensitizing target cells for lysis by a CTL clone at 1 nM peptide concentration. Although some modified peptides could be recognized by the CTL clone, none were found to be better recognized by T cells than the parental peptide. Like other melanoma differentiation antigens, TRP-2 was only expressed in melanoma, melanocytes, and retina, but not in other human tissues tested.

Original languageEnglish
Pages (from-to)2207-2216
Number of pages10
JournalJournal of Experimental Medicine
Volume184
Issue number6
DOIs
Publication statusPublished - 1996 Dec 1
Externally publishedYes

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Cytotoxic T-Lymphocytes
Neoplasm Antigens
Peptides
Clone Cells
Epitopes
Melanoma
Melanoma-Specific Antigens
Melanocytes
Differentiation Antigens
Interleukin-2
Retina
Complementary DNA
T-Lymphocytes
Cell Line
Genes
Neoplasms
HLA-A31 antigen

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of TRP-2 as a human tumor antigen recognized by cytotoxic T lymphocytes. / Wang, Rong Fu; Appella, Ettore; Kawakami, Yutaka; Kang, Xiaoqiang; Rosenberg, Steven A.

In: Journal of Experimental Medicine, Vol. 184, No. 6, 01.12.1996, p. 2207-2216.

Research output: Contribution to journalArticle

Wang, Rong Fu ; Appella, Ettore ; Kawakami, Yutaka ; Kang, Xiaoqiang ; Rosenberg, Steven A. / Identification of TRP-2 as a human tumor antigen recognized by cytotoxic T lymphocytes. In: Journal of Experimental Medicine. 1996 ; Vol. 184, No. 6. pp. 2207-2216.
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