Identification of TRP-2 as a human tumor antigen recognized by cytotoxic T lymphocytes

Rong Fu Wang, Ettore Appella, Yutaka Kawakami, Xiaoqiang Kang, Steven A. Rosenberg

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Abstract

The infusion of TIL586 along with interleukin-2 into the autologous patient with metastatic melanoma resulted in the objective regression of tumor. A gene encoding a tumor antigen recognized by TIL586 was previously isolated and shown to encode gp75 or TRP-1. Here we report that TRP-2 was identified as a second tumor antigen recognized by a HLA-A31-restricted CTL clone derived from the TIL586 cell line. The peptide LLPGGRPYR epitope was subsequently identified from the coding region of TRP-2 based on studies of the recognition of truncated TRP-2 cDNAs and the HLA-A31 binding motif. This epitope peptide was capable of sensitizing target cells for lysis by a CTL clone at 1 nM peptide concentration. Although some modified peptides could be recognized by the CTL clone, none were found to be better recognized by T cells than the parental peptide. Like other melanoma differentiation antigens, TRP-2 was only expressed in melanoma, melanocytes, and retina, but not in other human tissues tested.

Original languageEnglish
Pages (from-to)2207-2216
Number of pages10
JournalJournal of Experimental Medicine
Volume184
Issue number6
DOIs
Publication statusPublished - 1996 Dec 1

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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