TY - JOUR
T1 - Identification of two novel 5' noncoding exons in human MNB/DYRK gene and alternatively spliced transcripts
AU - Wang, Jun
AU - Kudoh, Jun
AU - Shintani, Ai
AU - Minoshima, Shinsei
AU - Shimizu, Nobuyoshi
N1 - Funding Information:
The authors thank N. Shindoh and H. Maeda for their contribution in the initial stage of this work, K. Kawasaki for helpful discussion, and Drs. Y. Sakaki and M. Hattori for the genomic sequence of MNB/DYRK gene region. This work was supported in part by Grants in Aid for Scientific Research on Priority Areas and Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan; and Fund for “Research for the Future” Program from the Japan Society for the Promotion of Science (JSPS).
PY - 1998/9/29
Y1 - 1998/9/29
N2 - Previously, the comparison of human MNB/DYRK cDNA sequences with publicly available genomic DNA sequence made a conclusion that MNB/DYRK gene consists of 11 exons spanning 100 kb. Here we found a novel human MNB/DYRK cDNA clone that has the 5' end different from any of those reported MNB/DYRK cDNAs. The 5' end of this cDNA resides in a CpG island associated with the NotI linking clone LL390 that is located more than 50 kb upstream of the exon that contains an initiation codon ATG. Northern blot analysis indicated that almost all the MNB/DYRK transcripts start from the newly identified 5'-noncoding exon. PCR amplification of cDNAs from various human tissues indicated that some mRNAs contain an additional noncoding exon of 76 bp next to the 5'-end exon and there are at least three types of MNB/DYRK mRNAs containing alternatively spliced 5' exons including two novel exons. We now conclude that human MNB/DYRK gene consists of 13 exons (not 11 exons) spanning about 150 kb (not 100 kb).
AB - Previously, the comparison of human MNB/DYRK cDNA sequences with publicly available genomic DNA sequence made a conclusion that MNB/DYRK gene consists of 11 exons spanning 100 kb. Here we found a novel human MNB/DYRK cDNA clone that has the 5' end different from any of those reported MNB/DYRK cDNAs. The 5' end of this cDNA resides in a CpG island associated with the NotI linking clone LL390 that is located more than 50 kb upstream of the exon that contains an initiation codon ATG. Northern blot analysis indicated that almost all the MNB/DYRK transcripts start from the newly identified 5'-noncoding exon. PCR amplification of cDNAs from various human tissues indicated that some mRNAs contain an additional noncoding exon of 76 bp next to the 5'-end exon and there are at least three types of MNB/DYRK mRNAs containing alternatively spliced 5' exons including two novel exons. We now conclude that human MNB/DYRK gene consists of 13 exons (not 11 exons) spanning about 150 kb (not 100 kb).
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U2 - 10.1006/bbrc.1998.9392
DO - 10.1006/bbrc.1998.9392
M3 - Article
C2 - 9784410
AN - SCOPUS:0032578584
SN - 0006-291X
VL - 250
SP - 704
EP - 710
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -