Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells

Tadayuki Akagi; Masayuki Usuda; Takahiko Matsuda; Minoru S.H. Ko; Hitoshi Niwa; Masahide Asano; Hiroshi Koide; Takashi Yokota

doi:10.1016/j.bbrc.2005.03.118

Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells

Tadayuki Akagi, Masayuki Usuda, Takahiko Matsuda, Minoru S.H. Ko, Hitoshi Niwa, Masahide Asano, Hiroshi Koide, Takashi Yokota

The Sakaguchi Laboratory of Developmental Biology Department of Systems Medicine

Research output: Contribution to journal › Article

34 Citations (Scopus)

Abstract

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.

Original language	English
Pages (from-to)	23-30
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	331
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.03.118
Publication status	Published - 2005 May 27

Keywords

ES cells
Oct-3/4
RNAi
STAT3
Self-renewal
Targeted disruption
Zfp-57

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Akagi, T., Usuda, M., Matsuda, T., Ko, M. S. H., Niwa, H., Asano, M., Koide, H., & Yokota, T. (2005). Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells. Biochemical and Biophysical Research Communications, 331(1), 23-30. https://doi.org/10.1016/j.bbrc.2005.03.118

Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells. / Akagi, Tadayuki; Usuda, Masayuki; Matsuda, Takahiko; Ko, Minoru S.H.; Niwa, Hitoshi; Asano, Masahide; Koide, Hiroshi; Yokota, Takashi.

In: Biochemical and Biophysical Research Communications, Vol. 331, No. 1, 27.05.2005, p. 23-30.

Research output: Contribution to journal › Article

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abstract = "Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.",

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