Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells

Tadayuki Akagi; Masayuki Usuda; Takahiko Matsuda; Minoru S.H. Ko; Hitoshi Niwa; Masahide Asano; Hiroshi Koide; Takashi Yokota

doi:10.1016/j.bbrc.2005.03.118

Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells

Tadayuki Akagi, Masayuki Usuda, Takahiko Matsuda, Minoru S.H. Ko, Hitoshi Niwa, Masahide Asano, Hiroshi Koide, Takashi Yokota

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.

Original language	English
Pages (from-to)	23-30
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	331
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.03.118
Publication status	Published - 2005 May 27
Externally published	Yes

Keywords

ES cells
Oct-3/4
RNAi
STAT3
Self-renewal
Targeted disruption
Zfp-57

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.03.118

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title = "Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells",

abstract = "Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.",

keywords = "ES cells, Oct-3/4, RNAi, STAT3, Self-renewal, Targeted disruption, Zfp-57",

author = "Tadayuki Akagi and Masayuki Usuda and Takahiko Matsuda and Ko, {Minoru S.H.} and Hitoshi Niwa and Masahide Asano and Hiroshi Koide and Takashi Yokota",

note = "Funding Information: We are grateful to Drs. Shinwa Shibata, Miwako Kajihara-Miura, and other members of our laboratory for their helpful discussions. We also thank the Center for Biomedical Research and Education at Kanazawa University for the use of the DNA sequencer. This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan.",

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AU - Akagi, Tadayuki

AU - Usuda, Masayuki

AU - Matsuda, Takahiko

AU - Ko, Minoru S.H.

AU - Niwa, Hitoshi

AU - Asano, Masahide

AU - Koide, Hiroshi

AU - Yokota, Takashi

N1 - Funding Information: We are grateful to Drs. Shinwa Shibata, Miwako Kajihara-Miura, and other members of our laboratory for their helpful discussions. We also thank the Center for Biomedical Research and Education at Kanazawa University for the use of the DNA sequencer. This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 2005/5/27

Y1 - 2005/5/27

N2 - Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.

AB - Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.

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Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells

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Keywords

ASJC Scopus subject areas

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