Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells

Tadayuki Akagi; Masayuki Usuda; Takahiko Matsuda; Minoru S.H. Ko; Hitoshi Niwa; Masahide Asano; Hiroshi Koide; Takashi Yokota

doi:10.1016/j.bbrc.2005.03.118

Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells

Tadayuki Akagi, Masayuki Usuda, Takahiko Matsuda, Minoru S.H. Ko, Hitoshi Niwa, Masahide Asano, Hiroshi Koide, Takashi Yokota

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.

Original language	English
Pages (from-to)	23-30
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	331
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.03.118
Publication status	Published - 2005 May 27
Externally published	Yes

Keywords

ES cells
Oct-3/4
RNAi
STAT3
Self-renewal
Targeted disruption
Zfp-57

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.03.118

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Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells. / Akagi, Tadayuki; Usuda, Masayuki; Matsuda, Takahiko; Ko, Minoru S.H.; Niwa, Hitoshi; Asano, Masahide; Koide, Hiroshi; Yokota, Takashi.

In: Biochemical and Biophysical Research Communications, Vol. 331, No. 1, 27.05.2005, p. 23-30.

Research output: Contribution to journal › Article › peer-review

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abstract = "Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.",

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author = "Tadayuki Akagi and Masayuki Usuda and Takahiko Matsuda and Ko, {Minoru S.H.} and Hitoshi Niwa and Masahide Asano and Hiroshi Koide and Takashi Yokota",

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AU - Yokota, Takashi

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N2 - Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.

AB - Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.

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Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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