Abstract
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.
Original language | English |
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Pages (from-to) | 23-30 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 331 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2005 May 27 |
Externally published | Yes |
Keywords
- ES cells
- Oct-3/4
- RNAi
- STAT3
- Self-renewal
- Targeted disruption
- Zfp-57
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology