Abstract
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.
| Original language | English |
|---|---|
| Pages (from-to) | 23-30 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 331 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2005 May 27 |
| Externally published | Yes |
Fingerprint
Keywords
- ES cells
- Oct-3/4
- RNAi
- Self-renewal
- STAT3
- Targeted disruption
- Zfp-57
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells. / Akagi, Tadayuki; Usuda, Masayuki; Matsuda, Takahiko; Ko, Minoru; Niwa, Hitoshi; Asano, Masahide; Koide, Hiroshi; Yokota, Takashi.
In: Biochemical and Biophysical Research Communications, Vol. 331, No. 1, 27.05.2005, p. 23-30.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells
AU - Akagi, Tadayuki
AU - Usuda, Masayuki
AU - Matsuda, Takahiko
AU - Ko, Minoru
AU - Niwa, Hitoshi
AU - Asano, Masahide
AU - Koide, Hiroshi
AU - Yokota, Takashi
PY - 2005/5/27
Y1 - 2005/5/27
N2 - Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.
AB - Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.
KW - ES cells
KW - Oct-3/4
KW - RNAi
KW - Self-renewal
KW - STAT3
KW - Targeted disruption
KW - Zfp-57
UR - http://www.scopus.com/inward/record.url?scp=17444417417&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17444417417&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.03.118
DO - 10.1016/j.bbrc.2005.03.118
M3 - Article
C2 - 15845352
AN - SCOPUS:17444417417
VL - 331
SP - 23
EP - 30
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -