Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells

Tadayuki Akagi, Masayuki Usuda, Takahiko Matsuda, Minoru Ko, Hitoshi Niwa, Masahide Asano, Hiroshi Koide, Takashi Yokota

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume331
Issue number1
DOIs
Publication statusPublished - 2005 May 27
Externally publishedYes

Fingerprint

Zinc Fingers
Embryonic Stem Cells
Stem cells
Zinc
Molecules
Proteins
Blastocyst Inner Cell Mass
STAT3 Transcription Factor
Oligonucleotide Array Sequence Analysis
Down-Regulation
Genes
Chemical activation
Defects
DNA

Keywords

  • ES cells
  • Oct-3/4
  • RNAi
  • Self-renewal
  • STAT3
  • Targeted disruption
  • Zfp-57

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells. / Akagi, Tadayuki; Usuda, Masayuki; Matsuda, Takahiko; Ko, Minoru; Niwa, Hitoshi; Asano, Masahide; Koide, Hiroshi; Yokota, Takashi.

In: Biochemical and Biophysical Research Communications, Vol. 331, No. 1, 27.05.2005, p. 23-30.

Research output: Contribution to journalArticle

Akagi, Tadayuki ; Usuda, Masayuki ; Matsuda, Takahiko ; Ko, Minoru ; Niwa, Hitoshi ; Asano, Masahide ; Koide, Hiroshi ; Yokota, Takashi. / Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 331, No. 1. pp. 23-30.
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