IL-1 is an important mediator for microcirculatory changes in endotoxin-induced intestinal mucosal damage

Dai Fukumura, Soichiro Miura, Iwao Kurose, Hajime Higuchi, Hidekazu Suzuki, Hirotoshi Ebinuma, Jing Yan Han, Naoyuki Watanabe, Go Wakabayashi, Masaki Kitajima, Hiromasa Ishii

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Although small intestine is frequently injured in endotoxin shock, the exact pathological sequence has not been fully understood. The major objective of this study is to elucidate the role of interleukin (IL)-1 in endotoxin-induced microcirculatory disturbance of rat small intestine. Mucosal and submucosal microvessels of the rat ileum were observed by intravital microscope with a high speed video camera system and the attenuating effect of E5090, an inhibitor of IL-1 generation, on endotoxin-induced intestinal microcirculatory disturbances was investigated. Endotoxin infusion produced significant mucosal damage, but before these morphological changes became significant, microvascular stasis in villi, decreased red blood cell velocity, and increased leukocyte adherence to venular walls were observed in intestinal microcirculatory beds 30 min after endotoxin administration. Intestinal IL-1 α levels were also significantly increased at that time. Endotoxin treatment enhanced chemiluminescence activity from neurophils and rapidly mobilized CD18 on leukocytes. E5090, which suppressed the IL-1 production in intestinal mucosa, attenuated the microcirculatory disturbances induced by endotoxin, and significantly reduced the subsequent mucosal damage. E5090 also attenuated the increased chemiluminescence activity and CD18 expression on leukocytes. In conclusion, the production of IL-1α is enhanced in the intestinal mucosa during endotoxin infusion. IL-1 may be an important mediator of microcirculatory changes, including decreased red blood cell velocity and increased leukocyte sticking and its activation, leading to the mucosal damage.

Original languageEnglish
Pages (from-to)2482-2492
Number of pages11
JournalDigestive Diseases and Sciences
Volume41
Issue number12
DOIs
Publication statusPublished - 1996

Fingerprint

Interleukin-1
Endotoxins
Leukocytes
Intestinal Mucosa
Luminescence
Small Intestine
Erythrocytes
Microvessels
Ileum
Shock
E 5090

Keywords

  • endotoxin
  • interleukin-1
  • intestinal mucosal damage
  • leukocyte sticking
  • microcirculatory disburbance
  • oxygen radicals

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Fukumura, D., Miura, S., Kurose, I., Higuchi, H., Suzuki, H., Ebinuma, H., ... Ishii, H. (1996). IL-1 is an important mediator for microcirculatory changes in endotoxin-induced intestinal mucosal damage. Digestive Diseases and Sciences, 41(12), 2482-2492. https://doi.org/10.1007/BF02100147

IL-1 is an important mediator for microcirculatory changes in endotoxin-induced intestinal mucosal damage. / Fukumura, Dai; Miura, Soichiro; Kurose, Iwao; Higuchi, Hajime; Suzuki, Hidekazu; Ebinuma, Hirotoshi; Han, Jing Yan; Watanabe, Naoyuki; Wakabayashi, Go; Kitajima, Masaki; Ishii, Hiromasa.

In: Digestive Diseases and Sciences, Vol. 41, No. 12, 1996, p. 2482-2492.

Research output: Contribution to journalArticle

Fukumura, D, Miura, S, Kurose, I, Higuchi, H, Suzuki, H, Ebinuma, H, Han, JY, Watanabe, N, Wakabayashi, G, Kitajima, M & Ishii, H 1996, 'IL-1 is an important mediator for microcirculatory changes in endotoxin-induced intestinal mucosal damage', Digestive Diseases and Sciences, vol. 41, no. 12, pp. 2482-2492. https://doi.org/10.1007/BF02100147
Fukumura, Dai ; Miura, Soichiro ; Kurose, Iwao ; Higuchi, Hajime ; Suzuki, Hidekazu ; Ebinuma, Hirotoshi ; Han, Jing Yan ; Watanabe, Naoyuki ; Wakabayashi, Go ; Kitajima, Masaki ; Ishii, Hiromasa. / IL-1 is an important mediator for microcirculatory changes in endotoxin-induced intestinal mucosal damage. In: Digestive Diseases and Sciences. 1996 ; Vol. 41, No. 12. pp. 2482-2492.
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