IL-10 produced by macrophages regulates epithelial integrity in the small intestine

Tina L. Morhardt, Atsushi Hayashi, Takanori Ochi, Miguel Quirós, Sho Kitamoto, Hiroko Nagao-Kitamoto, Peter Kuffa, Koji Atarashi, Kenya Honda, John Y. Kao, Asma Nusrat, Nobuhiko Kamada

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Macrophages (Mϕs) are known to be major producers of the anti-inflammatory cytokine interleukin-10 (IL-10) in the intestine, thus playing an important role in maintaining gastrointestinal homeostasis. Mϕs that reside in the small intestine (SI) have been previously shown to be regulated by dietary antigens, while colonic Mϕs are regulated by the microbiota. However, the role which resident Mϕs play in SI homeostasis has not yet been fully elucidated. Here, we show that SI Mϕs regulate the integrity of the epithelial barrier via secretion of IL-10. We used an animal model of non-steroidal anti-inflammatory drug (NSAID)-induced SI epithelial injury to show that IL-10 is mainly produced by MHCII+ CD64+ Ly6Clow Mϕs early in injury and that it is involved in the restoration of the epithelial barrier. We found that a lack of IL-10, particularly its secretion by Mϕs, compromised the recovery of SI epithelial barrier. IL-10 production by MHCII+ CD64+ Ly6Clow Mϕs in the SI is not regulated by the gut microbiota, hence depletion of the microbiota did not influence epithelial regeneration in the SI. Collectively, these results highlight the critical role IL-10-producing Mϕs play in recovery from intestinal epithelial injury induced by NSAID.

Original languageEnglish
Article number1223
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'IL-10 produced by macrophages regulates epithelial integrity in the small intestine'. Together they form a unique fingerprint.

  • Cite this

    Morhardt, T. L., Hayashi, A., Ochi, T., Quirós, M., Kitamoto, S., Nagao-Kitamoto, H., Kuffa, P., Atarashi, K., Honda, K., Kao, J. Y., Nusrat, A., & Kamada, N. (2019). IL-10 produced by macrophages regulates epithelial integrity in the small intestine. Scientific reports, 9(1), [1223]. https://doi.org/10.1038/s41598-018-38125-x