IL-10-producing CD4+ T cells negatively regulate fucosylation of epithelial cells in the gut

Yoshiyuki Goto, Aayam Lamichhane, Mariko Kamioka, Shintaro Sato, Kenya Honda, Jun Kunisawa, Hiroshi Kiyono

Research output: Contribution to journalArticle

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Abstract

Fucosylated glycans on the surface of epithelial cells (ECs) regulate intestinal homeostasis by serving as attachment receptors and a nutrient source for some species of bacteria. We show here that epithelial fucosylation in the ileum is negatively regulated by IL-10-producing CD4+ T cells. The number of fucosylated ECs was increased in the ileum of mice lacking T cells, especially those expressing αβ T cell receptor (TCR), CD4, and IL-10. No such effect was observed in mice lacking B cells. Adoptive transfer of αβTCR + CD4+ T cells from normal mice, but not IL-10-deficient mice, normalized fucosylation of ECs. These findings suggest that IL-10-producing CD4+ T cells contribute to the maintenance of the function of ECs by regulating their fucosylation.

Original languageEnglish
Article number15918
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2015 Nov 2
Externally publishedYes

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Interleukin-10
Epithelial Cells
T-Lymphocytes
T-Cell Antigen Receptor
Ileum
Adoptive Transfer
Polysaccharides
Homeostasis
B-Lymphocytes
Maintenance
Bacteria
Food

ASJC Scopus subject areas

  • General

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IL-10-producing CD4+ T cells negatively regulate fucosylation of epithelial cells in the gut. / Goto, Yoshiyuki; Lamichhane, Aayam; Kamioka, Mariko; Sato, Shintaro; Honda, Kenya; Kunisawa, Jun; Kiyono, Hiroshi.

In: Scientific Reports, Vol. 5, 15918, 02.11.2015.

Research output: Contribution to journalArticle

Goto, Yoshiyuki ; Lamichhane, Aayam ; Kamioka, Mariko ; Sato, Shintaro ; Honda, Kenya ; Kunisawa, Jun ; Kiyono, Hiroshi. / IL-10-producing CD4+ T cells negatively regulate fucosylation of epithelial cells in the gut. In: Scientific Reports. 2015 ; Vol. 5.
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