IL-2 is positively involved in the development of colitogenic CD4 + IL-7Rαhigh memory T cells in chronic colitis

Kaori Kameyama, Yasuhiro Nemoto, Takanori Kanai, Tamako Shinohara, Ryuichi Okamoto, Kiichiro Tsuchiya, Tetsuya Nakamura, Naoya Sakamoto, Teruji Totsuka, Toshifumi Hibi, Mamoru Watanabe

Research output: Contribution to journalArticle

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Abstract

IL-2 and IL-7 share a common γ-chain receptor and are critical for T-cell homeostasis. We aimed to clarify the reciprocal roles of IL-2 and IL-7 in the development and persistence of chronic colitis. We performed a series of adoptive transfers of IL-2-/- CD4+CD45RBhigh T cells into RAG-2-/- mice and assessed the role of IL-2 in the induction of IL-7Rα on colitogenic CD4+ T cells and the development of chronic colitis. RAG-2-/- mice transferred with WT but not with IL-2-/- CD4+CD45RBhigh T cells developed Th1/Th17-mediated colitis. Consistently, re-expression of IL-7Rα was severely impaired on IL-2-/- but not on WT CD4+ T cells from the transferred mice. To exclude a contribution of the preclinical autoimmunity of IL-2-/-mice, WT Ly5.1+ or IL-2 -/- Ly5.2+ CD4+CD45RBhigh T cells from GFP mice previously transplanted with the same number of WT and IL-2 -/- BM cells were transferred into RAG-2-/- mice. RAG-2-/- mice transferred with IL-2-/--derived CD4 + CD45RBhigh T cells did not develop colitis, but their splenic CD4+ T cells changed from effector-memory to central-memory type. These results show that IL-2 is critically involved in the establishment and maintenance of IL-7-dependent colitogenic memory CD4+IL- 7Rαhigh T cells.

Original languageEnglish
Pages (from-to)2423-2436
Number of pages14
JournalEuropean Journal of Immunology
Volume40
Issue number9
DOIs
Publication statusPublished - 2010

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Colitis
Interleukin-2
T-Lymphocytes
Interleukin-7
Adoptive Transfer
T-Cell Antigen Receptor
Autoimmunity
Homeostasis
Maintenance

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

Cite this

IL-2 is positively involved in the development of colitogenic CD4 + IL-7Rαhigh memory T cells in chronic colitis. / Kameyama, Kaori; Nemoto, Yasuhiro; Kanai, Takanori; Shinohara, Tamako; Okamoto, Ryuichi; Tsuchiya, Kiichiro; Nakamura, Tetsuya; Sakamoto, Naoya; Totsuka, Teruji; Hibi, Toshifumi; Watanabe, Mamoru.

In: European Journal of Immunology, Vol. 40, No. 9, 2010, p. 2423-2436.

Research output: Contribution to journalArticle

Kameyama, K, Nemoto, Y, Kanai, T, Shinohara, T, Okamoto, R, Tsuchiya, K, Nakamura, T, Sakamoto, N, Totsuka, T, Hibi, T & Watanabe, M 2010, 'IL-2 is positively involved in the development of colitogenic CD4 + IL-7Rαhigh memory T cells in chronic colitis', European Journal of Immunology, vol. 40, no. 9, pp. 2423-2436. https://doi.org/10.1002/eji.200939764
Kameyama, Kaori ; Nemoto, Yasuhiro ; Kanai, Takanori ; Shinohara, Tamako ; Okamoto, Ryuichi ; Tsuchiya, Kiichiro ; Nakamura, Tetsuya ; Sakamoto, Naoya ; Totsuka, Teruji ; Hibi, Toshifumi ; Watanabe, Mamoru. / IL-2 is positively involved in the development of colitogenic CD4 + IL-7Rαhigh memory T cells in chronic colitis. In: European Journal of Immunology. 2010 ; Vol. 40, No. 9. pp. 2423-2436.
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AU - Kanai, Takanori

AU - Shinohara, Tamako

AU - Okamoto, Ryuichi

AU - Tsuchiya, Kiichiro

AU - Nakamura, Tetsuya

AU - Sakamoto, Naoya

AU - Totsuka, Teruji

AU - Hibi, Toshifumi

AU - Watanabe, Mamoru

PY - 2010

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AB - IL-2 and IL-7 share a common γ-chain receptor and are critical for T-cell homeostasis. We aimed to clarify the reciprocal roles of IL-2 and IL-7 in the development and persistence of chronic colitis. We performed a series of adoptive transfers of IL-2-/- CD4+CD45RBhigh T cells into RAG-2-/- mice and assessed the role of IL-2 in the induction of IL-7Rα on colitogenic CD4+ T cells and the development of chronic colitis. RAG-2-/- mice transferred with WT but not with IL-2-/- CD4+CD45RBhigh T cells developed Th1/Th17-mediated colitis. Consistently, re-expression of IL-7Rα was severely impaired on IL-2-/- but not on WT CD4+ T cells from the transferred mice. To exclude a contribution of the preclinical autoimmunity of IL-2-/-mice, WT Ly5.1+ or IL-2 -/- Ly5.2+ CD4+CD45RBhigh T cells from GFP mice previously transplanted with the same number of WT and IL-2 -/- BM cells were transferred into RAG-2-/- mice. RAG-2-/- mice transferred with IL-2-/--derived CD4 + CD45RBhigh T cells did not develop colitis, but their splenic CD4+ T cells changed from effector-memory to central-memory type. These results show that IL-2 is critically involved in the establishment and maintenance of IL-7-dependent colitogenic memory CD4+IL- 7Rαhigh T cells.

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