IL-22BP dictates characteristics of Peyer's patch follicleassociated epithelium for antigen uptake

Toshi Jinnohara, Takashi Kanaya, Kouji Hase, Sayuri Sakakibara, Tamotsu Kato, Naoko Tachibana, Takaharu Sasaki, Yusuke Hashimoto, Toshiro Sato, Hiroshi Watarai, Jun Kunisawa, Naoko Shibata, Ifor R. Williams, Hiroshi Kiyono, Hiroshi Ohno

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Interleukin-22 (IL-22) acts protectively and harmfully on intestinal tissue depending on the situation; therefore, IL-22 signaling needs to be tightly regulated. IL-22 binding protein (IL-22BP) binds IL-22 to inhibit IL-22 signaling. It is expressed in intestinal and lymphoid tissues, although its precise distribution and roles have remained unclear. In this study, we show that IL-22BP is highly expressed by CD11b+CD8α- dendritic cells in the subepithelial dome region of Peyer's patches (PPs). We found that IL-22BP blocks IL-22 signaling in the follicle-associated epithelium (FAE) covering PPs, indicating that IL-22BP plays a role in regulating the characteristics of the FAE. As expected, FAE of IL-22BP-deficient (Il22ra2-/-) mice exhibited altered properties such as the enhanced expression of mucus and antimicrobial proteins as well as prominent fucosylation, which are normally suppressed in FAE. Additionally, Il22ra2-/- mice exhibited the decreased uptake of bacterial antigens into PPs without affecting M cell function. Our present study thus demonstrates that IL-22BP promotes bacterial uptake into PPs by influencing FAE gene expression and function.

Original languageEnglish
Pages (from-to)1607-1618
Number of pages12
JournalJournal of Experimental Medicine
Volume214
Issue number6
DOIs
Publication statusPublished - 2017 Jun 1

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Peyer's Patches
Carrier Proteins
Epithelium
Antigens
interleukin-22
Bacterial Antigens
Lymphoid Tissue
Mucus
Dendritic Cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

IL-22BP dictates characteristics of Peyer's patch follicleassociated epithelium for antigen uptake. / Jinnohara, Toshi; Kanaya, Takashi; Hase, Kouji; Sakakibara, Sayuri; Kato, Tamotsu; Tachibana, Naoko; Sasaki, Takaharu; Hashimoto, Yusuke; Sato, Toshiro; Watarai, Hiroshi; Kunisawa, Jun; Shibata, Naoko; Williams, Ifor R.; Kiyono, Hiroshi; Ohno, Hiroshi.

In: Journal of Experimental Medicine, Vol. 214, No. 6, 01.06.2017, p. 1607-1618.

Research output: Contribution to journalArticle

Jinnohara, T, Kanaya, T, Hase, K, Sakakibara, S, Kato, T, Tachibana, N, Sasaki, T, Hashimoto, Y, Sato, T, Watarai, H, Kunisawa, J, Shibata, N, Williams, IR, Kiyono, H & Ohno, H 2017, 'IL-22BP dictates characteristics of Peyer's patch follicleassociated epithelium for antigen uptake', Journal of Experimental Medicine, vol. 214, no. 6, pp. 1607-1618. https://doi.org/10.1084/jem.20160770
Jinnohara, Toshi ; Kanaya, Takashi ; Hase, Kouji ; Sakakibara, Sayuri ; Kato, Tamotsu ; Tachibana, Naoko ; Sasaki, Takaharu ; Hashimoto, Yusuke ; Sato, Toshiro ; Watarai, Hiroshi ; Kunisawa, Jun ; Shibata, Naoko ; Williams, Ifor R. ; Kiyono, Hiroshi ; Ohno, Hiroshi. / IL-22BP dictates characteristics of Peyer's patch follicleassociated epithelium for antigen uptake. In: Journal of Experimental Medicine. 2017 ; Vol. 214, No. 6. pp. 1607-1618.
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