IL-4 regulates chemokine production induced by TNF-α in keratocytes and corneal epithelial cells

Yoji Takano, Kazumi Fukagawa, Shigeto Shimmura, Kazuo Tsubota, Yoshihisa Oguchi, Hirohisa Saito

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background - Eosinophils are thought to play a major role in the pathogenesis of corneal lesions in ocular allergies. The regulation of chemokine production in corneal cells by the Th2 cytokine, interleukin 4 (IL-4), was examined in order to investigate its role in ocular allergies. Methods - Pure cultures of human corneal epithelial cells and keratocytes were exposed to tumour necrosis factor α (TNF-α) and/or IL-4. 24 hours after exposure, culture supernatants were removed and concentrations of IL-8 and RANTES were quantified by ELISA assay. Results - Simultaneous addition of IL-4 inhibited TNF-α induced IL-8 production in both corneal epithelial cells and keratocytes. TNF-α and IL-4 synergistically stimulated the production of RANTES in keratocytes. Conclusion - Differential regulation of chemokine production from corneal cells by IL-4 may play a role in the selective recruitment of predominantly eosinophils to the ocular surface in ocular allergies.

Original languageEnglish
Pages (from-to)1074-1076
Number of pages3
JournalBritish Journal of Ophthalmology
Volume83
Issue number9
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Corneal Keratocytes
Chemokines
Interleukin-4
Tumor Necrosis Factor-alpha
Epithelial Cells
Chemokine CCL5
Hypersensitivity
Interleukin-8
Eosinophils
Th2 Cells
Enzyme-Linked Immunosorbent Assay
Cytokines

ASJC Scopus subject areas

  • Ophthalmology

Cite this

IL-4 regulates chemokine production induced by TNF-α in keratocytes and corneal epithelial cells. / Takano, Yoji; Fukagawa, Kazumi; Shimmura, Shigeto; Tsubota, Kazuo; Oguchi, Yoshihisa; Saito, Hirohisa.

In: British Journal of Ophthalmology, Vol. 83, No. 9, 1999, p. 1074-1076.

Research output: Contribution to journalArticle

Takano, Yoji ; Fukagawa, Kazumi ; Shimmura, Shigeto ; Tsubota, Kazuo ; Oguchi, Yoshihisa ; Saito, Hirohisa. / IL-4 regulates chemokine production induced by TNF-α in keratocytes and corneal epithelial cells. In: British Journal of Ophthalmology. 1999 ; Vol. 83, No. 9. pp. 1074-1076.
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AU - Fukagawa, Kazumi

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AU - Tsubota, Kazuo

AU - Oguchi, Yoshihisa

AU - Saito, Hirohisa

PY - 1999

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N2 - Background - Eosinophils are thought to play a major role in the pathogenesis of corneal lesions in ocular allergies. The regulation of chemokine production in corneal cells by the Th2 cytokine, interleukin 4 (IL-4), was examined in order to investigate its role in ocular allergies. Methods - Pure cultures of human corneal epithelial cells and keratocytes were exposed to tumour necrosis factor α (TNF-α) and/or IL-4. 24 hours after exposure, culture supernatants were removed and concentrations of IL-8 and RANTES were quantified by ELISA assay. Results - Simultaneous addition of IL-4 inhibited TNF-α induced IL-8 production in both corneal epithelial cells and keratocytes. TNF-α and IL-4 synergistically stimulated the production of RANTES in keratocytes. Conclusion - Differential regulation of chemokine production from corneal cells by IL-4 may play a role in the selective recruitment of predominantly eosinophils to the ocular surface in ocular allergies.

AB - Background - Eosinophils are thought to play a major role in the pathogenesis of corneal lesions in ocular allergies. The regulation of chemokine production in corneal cells by the Th2 cytokine, interleukin 4 (IL-4), was examined in order to investigate its role in ocular allergies. Methods - Pure cultures of human corneal epithelial cells and keratocytes were exposed to tumour necrosis factor α (TNF-α) and/or IL-4. 24 hours after exposure, culture supernatants were removed and concentrations of IL-8 and RANTES were quantified by ELISA assay. Results - Simultaneous addition of IL-4 inhibited TNF-α induced IL-8 production in both corneal epithelial cells and keratocytes. TNF-α and IL-4 synergistically stimulated the production of RANTES in keratocytes. Conclusion - Differential regulation of chemokine production from corneal cells by IL-4 may play a role in the selective recruitment of predominantly eosinophils to the ocular surface in ocular allergies.

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