IL-4 regulates IL-2 induction of lymphokine-activated killer activity from human lymphocytes

Yutaka Kawakami, M. C. Custer, S. A. Rosenberg, M. T. Lotze

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

IL-4 is a pluripotent lymphokine acting on various cell types. We investigated the role of human IL-4 on the generation of lymphokine-activated killer (LAK) activity. Human IL-4 alone did not induce LAK activity and inhibited IL-2 induction of LAK activity from unstimulated PBMC, peripheral blood null cells, spleen cells, and lymph node cells in a dose-dependent manner. IL-4 also inhibited several phenomena induced by IL-2 such as cell proliferation, augmentation of NK activity, increase of Leu-19+ cells, and expression of IL-2R(p55) on either CD3+ or Leu-19+ cells. IL-4, however, augmented cell proliferation with other T cell mitogens including PHA, Con A, PMA, or allo-MHC Ag with or without IL-2. In contrast to unstimulated cells, IL-4 alone induced marked cell proliferation and LAK activity as well as Leu-19+ cells from in vitro IL-2 preactivated PBMC or null cells, and did not inhibit IL-2 induced cell proliferation, LAK activity, Leu-19+ cells and IL-2R(p55) expression, but rather augmented them with low doses of IL-2. Although IL-4 alone induced LAK activity from peripheral blood of some patients previously given IL-2, IL-4 inhibited in vitro LAK generation with IL-2 from these cells in most cases. Therefore, IL-4 appears to directly inhibit the IL-2 activation pathway via IL-2R(p70) and prevent resting LAK precursors from proliferating and differentiating into final effector cells. However, once cells were sufficiently preactivated by IL-2, IL-4 induced LAK activity and did not inhibit IL-2 activation of these cells. These data suggest an immunoregulatory role of IL-4 on human null cells and T cells.

Original languageEnglish
Pages (from-to)3452-3461
Number of pages10
JournalJournal of Immunology
Volume142
Issue number10
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Lymphokines
Human Activities
Interleukin-4
Interleukin-2
Lymphocytes
Null Lymphocytes
Cell Proliferation
T-Lymphocytes
Mitogens
Blood Cells
Spleen
Lymph Nodes

ASJC Scopus subject areas

  • Immunology

Cite this

Kawakami, Y., Custer, M. C., Rosenberg, S. A., & Lotze, M. T. (1989). IL-4 regulates IL-2 induction of lymphokine-activated killer activity from human lymphocytes. Journal of Immunology, 142(10), 3452-3461.

IL-4 regulates IL-2 induction of lymphokine-activated killer activity from human lymphocytes. / Kawakami, Yutaka; Custer, M. C.; Rosenberg, S. A.; Lotze, M. T.

In: Journal of Immunology, Vol. 142, No. 10, 1989, p. 3452-3461.

Research output: Contribution to journalArticle

Kawakami, Y, Custer, MC, Rosenberg, SA & Lotze, MT 1989, 'IL-4 regulates IL-2 induction of lymphokine-activated killer activity from human lymphocytes', Journal of Immunology, vol. 142, no. 10, pp. 3452-3461.
Kawakami, Yutaka ; Custer, M. C. ; Rosenberg, S. A. ; Lotze, M. T. / IL-4 regulates IL-2 induction of lymphokine-activated killer activity from human lymphocytes. In: Journal of Immunology. 1989 ; Vol. 142, No. 10. pp. 3452-3461.
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