IL-6-dependent and -independent pathways in the development of interleukin 17-producing T helper cells

Akihiro Kimura, Tetsuji Naka, Tadamitsu Kishimoto

Research output: Contribution to journalArticle

252 Citations (Scopus)

Abstract

CD4+ T cells producing IL-17 [T helper (Th)17], as distinct from Th1 or Th2 cells, have recently been shown to be associated with autoimmunity, but it is not entirely clear how Th17 cells are generated from naïve T cells. We demonstrate here that IL-6, but not TNF-α or IL-1β, can, in combination with TGF-β, induce Th17 cell generation from naïve T cells and inhibit TGF-β-induced Foxp3 expression. Moreover, conditioned medium from lipopolysaccharide-stimulated bone marrow-derived dendritic cells (DCCM) can induce IL-17 production in naïve T cells. Interestingly, IL-17 was produced by DCCM even with the addition of anti-gp130 antibody or DCCM from IL-6 KO mice. The combination of IL-6 and TGF-β could maintain activation of signal transducer and activator of transcription (Stat)3, but not of Stat1. IL-27 or IFN-γ suppressed the induction of Th17 cells by TGF-β plus IL-6 and maintained Stat1 activation under these conditions. In contrast, both Stat1 and Stat3 remained to be activated in naïve T cells cultured with DCCM. These findings represent a different basis for Th17 differentiation from naïve T cells.

Original languageEnglish
Pages (from-to)12099-12104
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number29
DOIs
Publication statusPublished - 2007 Jul 17

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Interleukin-17
Helper-Inducer T-Lymphocytes
Interleukin-6
T-Lymphocytes
Th17 Cells
Interleukin-27
STAT3 Transcription Factor
Th2 Cells
Th1 Cells
Conditioned Culture Medium
Autoimmunity
Interleukin-1
Dendritic Cells
Lipopolysaccharides
Anti-Idiotypic Antibodies
Bone Marrow

Keywords

  • γt
  • Regulatory T cells
  • STAT
  • TGF-β retinoid-related orphan receptor

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

IL-6-dependent and -independent pathways in the development of interleukin 17-producing T helper cells. / Kimura, Akihiro; Naka, Tetsuji; Kishimoto, Tadamitsu.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 29, 17.07.2007, p. 12099-12104.

Research output: Contribution to journalArticle

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