IL-6 induction in desiccated corneal epithelium in vitro and in vivo

Akihiro Higuchi, Tetsuya Kawakita, Kazuo Tsubota

Research output: Contribution to journalArticle

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Abstract

Purpose: To investigate the effect of desiccation on secretion of inflammatory cytokines in corneal epithelial cells and in the rat desiccation model. Methods: A human corneal epithelial cell line (CEPI) was grown in keratinocyte growth medium 2 (KGM2) to approximately 80% confluence. The medium was aspirated and dishes were left for 0 to 30 min with the cover left open to dry the cells (short-term desiccation). After desiccation, KGM2 was added to the dishes and collected from the dishes 15 min later to measure the concentrations of cytokines in the medium by sandwich enzyme immunoassay (ELISA). Viability of the cells was estimated with alamer blue. To study the effect of long-term desiccation, cultivated cells on transwells were used. After dessiccation for up to 8 h, the viability of the cells and levels of cytokines in the culture medium were examined. The expression of cytokines in the cornea of the dry eye model rat was measured by real-time PCR. Results: Short-term dessication of CEPI cells significantly increased the interleukin (IL)-6 level and slightly increased the tumor necrosis factor (TNF)-α level. Anti-IL-6 antibody partially suppressed cell death caused by desiccation. Upon long-term desiccation, IL-6 and IL-8 levels were increased. In the dry eye model rats, the IL-6 mRNA level in the cornea significantly increased, whereas TNF-α mRNA level slightly increased. Conclusions: Desiccation induced IL-6 expression in corneal epithelial cells, suggesting that IL-6 participates in desiccation-induced cell death.

Original languageEnglish
Pages (from-to)2400-2406
Number of pages7
JournalMolecular Vision
Volume17
Publication statusPublished - 2011 Sep 13

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Desiccation
Corneal Epithelium
Interleukin-6
Cytokines
Epithelial Cells
Keratinocytes
Cornea
Cell Survival
Cell Death
Tumor Necrosis Factor-alpha
In Vitro Techniques
Messenger RNA
Growth
Immunoenzyme Techniques
Interleukin-8
Culture Media
Real-Time Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay
Cell Line

ASJC Scopus subject areas

  • Ophthalmology

Cite this

IL-6 induction in desiccated corneal epithelium in vitro and in vivo. / Higuchi, Akihiro; Kawakita, Tetsuya; Tsubota, Kazuo.

In: Molecular Vision, Vol. 17, 13.09.2011, p. 2400-2406.

Research output: Contribution to journalArticle

Higuchi, Akihiro ; Kawakita, Tetsuya ; Tsubota, Kazuo. / IL-6 induction in desiccated corneal epithelium in vitro and in vivo. In: Molecular Vision. 2011 ; Vol. 17. pp. 2400-2406.
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