IL-7 receptor α+ CD3- cells in the embryonic intestine induces the organizing center of Peyer's patches

Hisahiro Yoshida, Kenya Honda, Reiko Shinkura, Satoko Adachi, Satomi Nishikawa, Kazushige Maki, Koichi Ikuta, Shin Ichi Nishikawa

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Abstract

Peyer's patch (PP) organogenesis proceeds through three histologically distinct steps: formation of organizing centers expressing VCAM-1 and ICAM-1 in segregated regions of the intestine at 15.5 days post-coitus (d.p.c.) (step I), accumulation of blood cells expressing different sets of surface markers to this region at 16.5-17.0 d.p.c. (step II), and entry of CD3+ and B220+ lymphocytes just before birth (step III). PP formation of both II7ra(-/-) and Lta(-/-) mice is impaired from step I, suggesting involvement of the two molecules at the same timing in PP organogenesis. Expression of lymphotoxin (LT) α and LTβ in IL-7 receptor (IL-7R) α+ cells in the intestine indicates that defects of II7ra(-/-) and Lta(-/-) mice are due to functional inability of IL-7Rα+ cells in the induction of PP anlage. Blocking of IL-7Rα function by a single injection of the antagonistic mAb in 15.5 d.p.c. embryos suppressed appearance of VCAM-1+ spots and expression of LTα and LTβ in the intestine, which eventually resulted in mice without PP but are otherwise normal, Intestinal IL-7Rα+ cells are lymphoid in morphology but CD3- and functional in both nu/nu and Rag2(-/-) mice. These results implicate IL-7Rα+ CD3- cells as the direct inducer of the organizing center of PP.

Original languageEnglish
Pages (from-to)643-655
Number of pages13
JournalInternational immunology
Volume11
Issue number5
DOIs
Publication statusPublished - 1999 Jun 1
Externally publishedYes

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Keywords

  • Development
  • IL-7Rα
  • Lymphotoxin
  • Organizing center
  • Peyer's patch

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Yoshida, H., Honda, K., Shinkura, R., Adachi, S., Nishikawa, S., Maki, K., Ikuta, K., & Nishikawa, S. I. (1999). IL-7 receptor α+ CD3- cells in the embryonic intestine induces the organizing center of Peyer's patches. International immunology, 11(5), 643-655. https://doi.org/10.1093/intimm/11.5.643