Imaging mass spectrometry revealed the production of lyso-phosphatidylcholine in the injured ischemic rat brain

S. Koizumi, S. Yamamoto, T. Hayasaka, Y. Konishi, M. Yamaguchi-Okada, N. Goto-Inoue, Yuki Sugiura, M. Setou, H. Namba

Research output: Contribution to journalArticle

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Abstract

To develop an effective neuroprotective strategy against ischemic injury, it is important to identify the key molecules involved in the progression of injury. Direct molecular analysis of tissue using mass spectrometry (MS) is a subject of much interest in the field of metabolomics. Most notably, imaging mass spectrometry (IMS) allows visualization of molecular distributions on the tissue surface. To understand lipid dynamics during ischemic injury, we performed IMS analysis on rat brain tissue sections with focal cerebral ischemia. Sprague-Dawley rats were sacrificed at 24 h after middle cerebral artery occlusion, and brain sections were prepared. IMS analyses were conducted using matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) in positive ion mode. To determine the molecular structures, the detected ions were subjected to tandem MS. The intensity counts of the ion signals of m/z 798.5 and m/z 760.5 that are revealed to be a phosphatidylcholine, PC (16:0/18:1) are reduced in the area of focal cerebral ischemia as compared to the normal cerebral area. In contrast, the signal of m/z 496.3, identified as a lyso-phosphatidylcholine, LPC (16:0), was clearly increased in the area of focal cerebral ischemia. In IMS analyses, changes of PC (16:0/18:1) and LPC (16:0) are observed beyond the border of the injured area. Together with previous reports-that PCs are hydrolyzed by phospholipase A 2 (PLA 2) and produce LPCs,-our present results suggest that LPC (16:0) is generated during the injury process after cerebral ischemia, presumably via PLA 2 activation, and that PC (16:0/18:1) is one of its precursor molecules.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalNeuroscience
Volume168
Issue number1
DOIs
Publication statusPublished - 2010 Jun
Externally publishedYes

Fingerprint

Phosphatidylcholines
Mass Spectrometry
Brain Ischemia
Brain
Phospholipases A
Wounds and Injuries
Ions
Metabolomics
Middle Cerebral Artery Infarction
Tissue Distribution
Tandem Mass Spectrometry
Molecular Structure
Sprague Dawley Rats
Lasers
Lipids
PC 16

Keywords

  • Brain imaging
  • Focal ischemia
  • Imaging mass spectrometry
  • Lyso-phosphatidylcholine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Koizumi, S., Yamamoto, S., Hayasaka, T., Konishi, Y., Yamaguchi-Okada, M., Goto-Inoue, N., ... Namba, H. (2010). Imaging mass spectrometry revealed the production of lyso-phosphatidylcholine in the injured ischemic rat brain. Neuroscience, 168(1), 219-225. https://doi.org/10.1016/j.neuroscience.2010.03.056

Imaging mass spectrometry revealed the production of lyso-phosphatidylcholine in the injured ischemic rat brain. / Koizumi, S.; Yamamoto, S.; Hayasaka, T.; Konishi, Y.; Yamaguchi-Okada, M.; Goto-Inoue, N.; Sugiura, Yuki; Setou, M.; Namba, H.

In: Neuroscience, Vol. 168, No. 1, 06.2010, p. 219-225.

Research output: Contribution to journalArticle

Koizumi, S, Yamamoto, S, Hayasaka, T, Konishi, Y, Yamaguchi-Okada, M, Goto-Inoue, N, Sugiura, Y, Setou, M & Namba, H 2010, 'Imaging mass spectrometry revealed the production of lyso-phosphatidylcholine in the injured ischemic rat brain', Neuroscience, vol. 168, no. 1, pp. 219-225. https://doi.org/10.1016/j.neuroscience.2010.03.056
Koizumi, S. ; Yamamoto, S. ; Hayasaka, T. ; Konishi, Y. ; Yamaguchi-Okada, M. ; Goto-Inoue, N. ; Sugiura, Yuki ; Setou, M. ; Namba, H. / Imaging mass spectrometry revealed the production of lyso-phosphatidylcholine in the injured ischemic rat brain. In: Neuroscience. 2010 ; Vol. 168, No. 1. pp. 219-225.
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