TY - JOUR
T1 - Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver
AU - Miyamura, Norio
AU - Nakamura, Takashi
AU - Goto-Inoue, Naoko
AU - Zaima, Nobuhiro
AU - Hayasaka, Takahiro
AU - Yamasaki, Tokiwa
AU - Terai, Shuji
AU - Sakaida, Isao
AU - Setou, Mitsutoshi
AU - Nishina, Hiroshi
N1 - Funding Information:
This work was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan , the Ministry of Health, Labour and Welfare of Japan , and the Japan Society for the Promotion of Science .
PY - 2011/4/29
Y1 - 2011/4/29
N2 - After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.
AB - After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.
KW - Imaging mass spectrometry
KW - Liver regeneration
KW - MALDI-TOF
KW - Metabolite imaging
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U2 - 10.1016/j.bbrc.2011.03.133
DO - 10.1016/j.bbrc.2011.03.133
M3 - Article
C2 - 21463609
AN - SCOPUS:79955441867
SN - 0006-291X
VL - 408
SP - 120
EP - 125
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -