Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver

Norio Miyamura, Takashi Nakamura, Naoko Goto-Inoue, Nobuhiro Zaima, Takahiro Hayasaka, Tokiwa Yamasaki, Shuji Terai, Isao Sakaida, Mitsutoshi Setou, Hiroshi Nishina

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.

Original languageEnglish
Pages (from-to)120-125
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume408
Issue number1
DOIs
Publication statusPublished - 2011 Apr 29
Externally publishedYes

Fingerprint

Liver
Mass spectrometry
Mass Spectrometry
Phospholipids
Triglycerides
Thin layer chromatography
Imaging techniques
Hepatectomy
Thin Layer Chromatography
Lipids
Adipose Tissue
Tissue
Phosphatidylcholines
Lipid Metabolism
Real-Time Polymerase Chain Reaction
Genes
Gene Expression

Keywords

  • Imaging mass spectrometry
  • Liver regeneration
  • MALDI-TOF
  • Metabolite imaging

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver. / Miyamura, Norio; Nakamura, Takashi; Goto-Inoue, Naoko; Zaima, Nobuhiro; Hayasaka, Takahiro; Yamasaki, Tokiwa; Terai, Shuji; Sakaida, Isao; Setou, Mitsutoshi; Nishina, Hiroshi.

In: Biochemical and Biophysical Research Communications, Vol. 408, No. 1, 29.04.2011, p. 120-125.

Research output: Contribution to journalArticle

Miyamura, Norio ; Nakamura, Takashi ; Goto-Inoue, Naoko ; Zaima, Nobuhiro ; Hayasaka, Takahiro ; Yamasaki, Tokiwa ; Terai, Shuji ; Sakaida, Isao ; Setou, Mitsutoshi ; Nishina, Hiroshi. / Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 408, No. 1. pp. 120-125.
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