Imaging mass spectrometry technology and application on ganglioside study; visualization of age-dependent accumulation of C20-ganglioside molecular species in the mouse hippocampus

Yuki Sugiura, Shuichi Shimma, Yoshiyuki Konishi, Maki K. Yamada, Mitsutoshi Setou

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Gangliosides are particularly abundant in the central nervous system (CNS) and thought to play important roles in memory formation, neuritogenesis, synaptic transmission, and other neural functions. Although several molecular species of gangliosides have been characterized and their individual functions elucidated, their differential distribution in the CNS are not well understood. In particular, whether the different molecular species show different distribution patterns in the brain remains unclear. We report the distinct and characteristic distributions of ganglioside molecular species, as revealed by imaging mass spectrometry (IMS). This technique can discriminate the molecular species, raised from both oligosaccharide and ceramide structure by determining the difference of the mass-to-charge ratio, and structural analysis by tandem mass spectrometry. Gangliosides in the CNS are characterized by the structure of the long-chain base (LCB) in the ceramide moiety. The LCB of the main ganglioside species has either 18 or 20 carbons (i.e., C18- or C20-sphingosine); we found that these 2 types of gangliosides are differentially distributed in the mouse brain. While the C18-species was widely distributed throughout the frontal brain, the C20-species selectively localized along the entorhinal-hippocampus projections, especially in the molecular layer (ML) of the dentate gyrus (DG). We revealed development- and aging-related accumulation of the C-20 species in the ML-DG. Thus it is possible to consider that this brain-region specific regulation of LCB chain length is particularly important for the distinct function in cells of CNS.

Original languageEnglish
Article numbere3232
JournalPLoS One
Volume3
Issue number9
DOIs
Publication statusPublished - 2008 Sep 18
Externally publishedYes

Fingerprint

gangliosides
Gangliosides
hippocampus
Mass spectrometry
Hippocampus
Mass Spectrometry
Visualization
Neurology
image analysis
mass spectrometry
Technology
Imaging techniques
central nervous system
Brain
mice
Central Nervous System
brain
ceramides
Ceramides
Dentate Gyrus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Imaging mass spectrometry technology and application on ganglioside study; visualization of age-dependent accumulation of C20-ganglioside molecular species in the mouse hippocampus. / Sugiura, Yuki; Shimma, Shuichi; Konishi, Yoshiyuki; Yamada, Maki K.; Setou, Mitsutoshi.

In: PLoS One, Vol. 3, No. 9, e3232, 18.09.2008.

Research output: Contribution to journalArticle

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