Imaging membrane potential in dendritic spines

Mutsuo Nuriya, Jiang Jiang, Boaz Nemet, Kenneth B. Eisenthal, Rafael Yuste

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Dendritic spines mediate most excitatory inputs in the brain. Although it is clear that spines compartmentalize calcium, it is still unknown what role, if any, they play in integrating synaptic inputs. To investigate the electrical function of spines directly, we used second harmonic generation (SHG) imaging of membrane potential in pyramidal neurons from hippocampal cultures and neocortical brain slices. With FM 4-64 as an intracellular SHG chromophore, we imaged membrane potential in the soma, dendritic branches, and spines. The SHG response to voltage was linear and seemed based on an electro-optic mechanism. The SHG sensitivity of the chromophore in spines was similar to that of the parent dendritic shaft and the soma. Backpropagation of somatic action potentials generated SHG signals at spines with similar amplitude and kinetics to somatic ones. Our optical measurements of membrane potential from spines demonstrate directly that backpropagating action potentials invade the spines.

Original languageEnglish
Pages (from-to)786-790
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number3
DOIs
Publication statusPublished - 2006 Jan 17
Externally publishedYes

Fingerprint

Dendritic Spines
Membrane Potentials
Spine
Carisoprodol
Action Potentials
Pyramidal Cells
Brain
Calcium

Keywords

  • Action potential
  • Backpropagation
  • Cortex
  • Pyramidal
  • Second-harmonic imaging

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Imaging membrane potential in dendritic spines. / Nuriya, Mutsuo; Jiang, Jiang; Nemet, Boaz; Eisenthal, Kenneth B.; Yuste, Rafael.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 3, 17.01.2006, p. 786-790.

Research output: Contribution to journalArticle

Nuriya, Mutsuo ; Jiang, Jiang ; Nemet, Boaz ; Eisenthal, Kenneth B. ; Yuste, Rafael. / Imaging membrane potential in dendritic spines. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 3. pp. 786-790.
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