Imaging of astrocytic activity in living rodents

Norio Takata; Yoshiaki Shinohara; Masamichi Ohkura; Tsuneko Mishima; Junichi Nakai; Hajime Hirase

doi:10.1007/978-1-62703-785-3_12

Imaging of astrocytic activity in living rodents

Norio Takata, Yoshiaki Shinohara, Masamichi Ohkura, Tsuneko Mishima, Junichi Nakai, Hajime Hirase

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Citations (Scopus)

Abstract

Mounting evidence from in vitro experiments supports bidirectional interactions between astrocytes and neurons, suggesting glial involvement in neuronal information processing in the brain. Elevation of the cytosolic calcium ion (Ca²⁺) concentration has been suggested to be important for gliotransmission; however, the study of Ca²⁺ dynamics in cerebral cortical astrocytes in vivo became possible only recently. Here, we describe a set of procedures to monitor Ca²⁺ concentration fluctuations in a population of astrocytes or in astrocytic processes using in vivo two-photon microscopy. Simultaneous recording of neuronal activity by electrodes and astrocytic activity by imaging is a promising way to reveal the nature of neuron-glia interactions in the brain of living rodents.

Original language	English
Title of host publication	Optical Imaging of Neocortical Dynamics
Publisher	Humana Press Inc.
Pages	191-207
Number of pages	17
ISBN (Print)	9781627037846
DOIs	https://doi.org/10.1007/978-1-62703-785-3_12
Publication status	Published - 2014
Externally published	Yes

Publication series

Name	Neuromethods
Volume	85
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Keywords

Adenovirus
Astrocytes
Astrocytic processes
Bolus loading
Cerebral cortex
G-CaMP

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)
Psychiatry and Mental health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-62703-785-3_12

Cite this

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T1 - Imaging of astrocytic activity in living rodents

AU - Takata, Norio

AU - Shinohara, Yoshiaki

AU - Ohkura, Masamichi

AU - Mishima, Tsuneko

AU - Nakai, Junichi

AU - Hirase, Hajime

PY - 2014

Y1 - 2014

N2 - Mounting evidence from in vitro experiments supports bidirectional interactions between astrocytes and neurons, suggesting glial involvement in neuronal information processing in the brain. Elevation of the cytosolic calcium ion (Ca2+) concentration has been suggested to be important for gliotransmission; however, the study of Ca2+ dynamics in cerebral cortical astrocytes in vivo became possible only recently. Here, we describe a set of procedures to monitor Ca2+ concentration fluctuations in a population of astrocytes or in astrocytic processes using in vivo two-photon microscopy. Simultaneous recording of neuronal activity by electrodes and astrocytic activity by imaging is a promising way to reveal the nature of neuron-glia interactions in the brain of living rodents.

AB - Mounting evidence from in vitro experiments supports bidirectional interactions between astrocytes and neurons, suggesting glial involvement in neuronal information processing in the brain. Elevation of the cytosolic calcium ion (Ca2+) concentration has been suggested to be important for gliotransmission; however, the study of Ca2+ dynamics in cerebral cortical astrocytes in vivo became possible only recently. Here, we describe a set of procedures to monitor Ca2+ concentration fluctuations in a population of astrocytes or in astrocytic processes using in vivo two-photon microscopy. Simultaneous recording of neuronal activity by electrodes and astrocytic activity by imaging is a promising way to reveal the nature of neuron-glia interactions in the brain of living rodents.

KW - Adenovirus

KW - Astrocytes

KW - Astrocytic processes

KW - Bolus loading

KW - Cerebral cortex

KW - G-CaMP

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BT - Optical Imaging of Neocortical Dynamics

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ER -

Imaging of astrocytic activity in living rodents

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

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