Imaging of electrochemical enzyme sensor on gold electrode using surface plasmon resonance

Yuzuru Iwasaki, Tatsuya Tobita, Kazuyoshi Kurihara, Tsutomu Horiuchi, Koji Suzuki, Osamu Niwa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Three types of imaging, namely layer structure, electrochemical reaction, and enzyme sensor response, were achieved by applying surface plasmon resonance (SPR) measurement to an electrochemical biosensor. We constructed glucose oxidase based mediator type sensors on a gold electrode by spotting the mediator that contained horseradish peroxidase and spin coating the glucose oxidase film. The layer structure of the sensor was imaged by means of angle scanning SPR measurement. The single sensor spot (about 1 mm in diameter) consisted of about 100×100 pixels and its spatial structure was imaged. The multilayer structure of the enzyme sensor had a complex reflectance-incident angle curve and this required us to choose a suitable incident angle for mapping the redox state. We chose an incident angle that provided the most significant reflection intensity difference by using data obtained from two angle scanning SPR measurements at different electrode potentials. At this incident angle, we controlled the electrochemical states of the spotted mediator in cyclic voltammetry and imaged the degree to which the charged site density changed. Finally, we mapped the enzymatic activity around the mediator spot by the enzymatic reoxidation of pre-reduced mediator in the presence of glucose.

Original languageEnglish
Pages (from-to)783-788
Number of pages6
JournalBiosensors and Bioelectronics
Volume17
Issue number9
DOIs
Publication statusPublished - 2002 Sep 1

Keywords

  • Enzyme
  • Glucose
  • HRP
  • Imaging
  • Os
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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