Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease

Hiromasa Takaishi, Takahiro Matsuki, Atsushi Nakazawa, Toshihiko Takada, Shoichi Kado, Takashi Asahara, Nobuhiko Kamada, Atsushi Sakuraba, Tomoharu Yajima, Hajime Higuchi, Nagamu Inoue, Haruhiko Ogata, Yasushi Iwao, Koji Nomoto, Ryuichiro Tanaka, Toshifumi Hibi

Research output: Contribution to journalArticle

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Abstract

Since genetically engineered animal models of inflammatory bowel disease (IBD) do not develop colitis under germ-free conditions, the intestinal microflora is thought to be one of the most important environmental factors associated with IBD. To understand the involvement of intestinal microflora in the pathogenesis of IBD, we analyzed the constituents of intestinal microflora in IBD. Faecal samples from 73 patients with ulcerative colitis (UC) and 23 patients with Crohn's disease (CD) were analyzed by quantitative PCR using 16S rRNA gene-targeted group-specific primers for Bacteroides fragilis group, Bifidobacterium, Clostridium coccoides groups, Clostridium leptum subgroup, Atopobium cluster, and seven species of Bacteroides. We analyzed the distribution of the predominant microflora by fluorescence in situ hybridization (FISH) using group-specific probes. We also examined the concentration of faecal organic acids produced by intestinal microflora. Contrary to previous reports, we found that the B. fragilis group was significantly decreased in the faeces of patients with IBD. Moreover, B. vulgatus was the predominant microflora in healthy controls and relatively decreased among IBD patients. Most of the microflora adhering to the colonic mucosa surrounding the mucus layer comprised C. coccoides group and Bifidobacterium. B. fragilis group mainly inhabited the faeces, but did not adhere to or invade the mucosa. The concentrations of propionic and butyric acids in the faeces were significantly decreased in patients with IBD. These findings indicate that IBD is not caused by a specific intestinal bacterial cluster or species and that disordered intestinal microflora could be involved in the pathogenesis of IBD.

Original languageEnglish
Pages (from-to)463-472
Number of pages10
JournalInternational Journal of Medical Microbiology
Volume298
Issue number5-6
DOIs
Publication statusPublished - 2008 Jul 1

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Constitution and Bylaws
Inflammatory Bowel Diseases
Bacteroides fragilis
Feces
Bifidobacterium
Clostridium
Mucous Membrane
Gastrointestinal Microbiome
Genetically Modified Animals
Bacteroides
Propionates
Colitis
Mucus
Fluorescence In Situ Hybridization
Ulcerative Colitis
rRNA Genes
Crohn Disease
Animal Models
Polymerase Chain Reaction
Acids

Keywords

  • 16s rRNA
  • Bacteroides fragilis group
  • Inflammatory bowel disease
  • Intestinal microflora
  • Organic acid

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease. / Takaishi, Hiromasa; Matsuki, Takahiro; Nakazawa, Atsushi; Takada, Toshihiko; Kado, Shoichi; Asahara, Takashi; Kamada, Nobuhiko; Sakuraba, Atsushi; Yajima, Tomoharu; Higuchi, Hajime; Inoue, Nagamu; Ogata, Haruhiko; Iwao, Yasushi; Nomoto, Koji; Tanaka, Ryuichiro; Hibi, Toshifumi.

In: International Journal of Medical Microbiology, Vol. 298, No. 5-6, 01.07.2008, p. 463-472.

Research output: Contribution to journalArticle

Takaishi, H, Matsuki, T, Nakazawa, A, Takada, T, Kado, S, Asahara, T, Kamada, N, Sakuraba, A, Yajima, T, Higuchi, H, Inoue, N, Ogata, H, Iwao, Y, Nomoto, K, Tanaka, R & Hibi, T 2008, 'Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease', International Journal of Medical Microbiology, vol. 298, no. 5-6, pp. 463-472. https://doi.org/10.1016/j.ijmm.2007.07.016
Takaishi, Hiromasa ; Matsuki, Takahiro ; Nakazawa, Atsushi ; Takada, Toshihiko ; Kado, Shoichi ; Asahara, Takashi ; Kamada, Nobuhiko ; Sakuraba, Atsushi ; Yajima, Tomoharu ; Higuchi, Hajime ; Inoue, Nagamu ; Ogata, Haruhiko ; Iwao, Yasushi ; Nomoto, Koji ; Tanaka, Ryuichiro ; Hibi, Toshifumi. / Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease. In: International Journal of Medical Microbiology. 2008 ; Vol. 298, No. 5-6. pp. 463-472.
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