Abstract
We studied the dependence of immediate myocardial cell response on irradiance and photosensitizer concentration by an extracellular photosensitization reaction. We have proposed a new methodology for tachyarrhythmia treatment using the extracellular photosensitization reaction. Immediate cell response is needed in the tachyarrhythmia treatment since electrical physiological diagnosis is performed during the operation to judge the treatment effect. Intracellular Ca<sup>2+</sup> during and after the reaction was measured using a confocal microscope and Fluo-4 AM as Ca<sup>2+</sup> dye. The photosensitizer concentration, irradiance, and radiant exposure were varied 10-30 μg/ml, 30-293 mW/cm<sup>2</sup>, and 10-40 J/cm<sup>2</sup>, respectively. We found that the necrosis occurrence timing after the laser irradiation beginning was decreased with the photosensitizer concentration and irradiance increasing. The minimum necrosis occurrence timing was 209 s with 30 μg/ml in photosensitizer concentration and 293 mW/cm<sup>2</sup> in irradiance.
| Original language | English |
|---|---|
| Pages (from-to) | O-70-O-71 |
| Journal | Transactions of Japanese Society for Medical and Biological Engineering |
| Volume | 52 |
| DOIs | |
| Publication status | Published - 2014 Aug 17 |
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Keywords
- Biological Engineering
- Medical Engineering
- Paper format
ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Immediate electrical physiological damage dependence of myocardial cells on irradiance and photosensitizer concentration. / Ogawa, Emiyu; Arai, Tsunenori.
In: Transactions of Japanese Society for Medical and Biological Engineering, Vol. 52, 17.08.2014, p. O-70-O-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immediate electrical physiological damage dependence of myocardial cells on irradiance and photosensitizer concentration
AU - Ogawa, Emiyu
AU - Arai, Tsunenori
PY - 2014/8/17
Y1 - 2014/8/17
N2 - We studied the dependence of immediate myocardial cell response on irradiance and photosensitizer concentration by an extracellular photosensitization reaction. We have proposed a new methodology for tachyarrhythmia treatment using the extracellular photosensitization reaction. Immediate cell response is needed in the tachyarrhythmia treatment since electrical physiological diagnosis is performed during the operation to judge the treatment effect. Intracellular Ca2+ during and after the reaction was measured using a confocal microscope and Fluo-4 AM as Ca2+ dye. The photosensitizer concentration, irradiance, and radiant exposure were varied 10-30 μg/ml, 30-293 mW/cm2, and 10-40 J/cm2, respectively. We found that the necrosis occurrence timing after the laser irradiation beginning was decreased with the photosensitizer concentration and irradiance increasing. The minimum necrosis occurrence timing was 209 s with 30 μg/ml in photosensitizer concentration and 293 mW/cm2 in irradiance.
AB - We studied the dependence of immediate myocardial cell response on irradiance and photosensitizer concentration by an extracellular photosensitization reaction. We have proposed a new methodology for tachyarrhythmia treatment using the extracellular photosensitization reaction. Immediate cell response is needed in the tachyarrhythmia treatment since electrical physiological diagnosis is performed during the operation to judge the treatment effect. Intracellular Ca2+ during and after the reaction was measured using a confocal microscope and Fluo-4 AM as Ca2+ dye. The photosensitizer concentration, irradiance, and radiant exposure were varied 10-30 μg/ml, 30-293 mW/cm2, and 10-40 J/cm2, respectively. We found that the necrosis occurrence timing after the laser irradiation beginning was decreased with the photosensitizer concentration and irradiance increasing. The minimum necrosis occurrence timing was 209 s with 30 μg/ml in photosensitizer concentration and 293 mW/cm2 in irradiance.
KW - Biological Engineering
KW - Medical Engineering
KW - Paper format
UR - http://www.scopus.com/inward/record.url?scp=84939452300&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84939452300&partnerID=8YFLogxK
U2 - 10.11239/jsmbe.52.O-70
DO - 10.11239/jsmbe.52.O-70
M3 - Article
AN - SCOPUS:84939452300
VL - 52
SP - O-70-O-71
JO - BME = Bio medical engineering / henshu, Nihon ME Gakkai
JF - BME = Bio medical engineering / henshu, Nihon ME Gakkai
SN - 1347-443X
ER -