Immediate response and cytotoxicity effect on myocardial cells by extracellular photosensitization reaction varying irradiance

E. Ogawa, A. Ito, T. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigated the extracellular photosensitization effect on myocardial cells using talaporfin sodium at various irradiance measuring cell lethality dependence on talaporfin sodium concentration and immediate response of intracellular Ca2+ as a fundamental study of the application for tachyarrhythmia treatment. The myocardial cell lethality was measured 2 h after the photosensitization reaction by WST assay varying the irradiance from 0.12 to 0.66 W/cm2. The intracellular Ca2+ of myocardial cell was observed during and until 10 min after the extracellular photosensitization reaction using fluo-4 AM and a confocal microscope varying the irradiance. The linear increasing behavior of the myocardial cell lethality with talaporfin sodium concentration was similarly obtained in the case of 0.12 and 0.29 W/cm2 in irradiance. The photocytotoxicity was not obtained in the case of 0.46 and 0.66 W/cm2 in irradiance. The time response of necrosis occurrence after the beginning of the extracellular photosensitization reaction was decreased with the irradiance increasing. We prospect that it may be caused by oxygen depletion in our in vitro system using 96 well plate since the irradiance and talaporfin sodium concentration were higher comparing the in vitro conditions of the general PDT for cancer.

Original languageEnglish
Title of host publication2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages5316-5319
Number of pages4
ISBN (Electronic)9781424479290
DOIs
Publication statusPublished - 2014 Nov 2
Event2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014 - Chicago, United States
Duration: 2014 Aug 262014 Aug 30

Publication series

Name2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014

Other

Other2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014
CountryUnited States
CityChicago
Period14/8/2614/8/30

ASJC Scopus subject areas

  • Health Informatics
  • Computer Science Applications
  • Biomedical Engineering
  • Medicine(all)

Fingerprint Dive into the research topics of 'Immediate response and cytotoxicity effect on myocardial cells by extracellular photosensitization reaction varying irradiance'. Together they form a unique fingerprint.

  • Cite this

    Ogawa, E., Ito, A., & Arai, T. (2014). Immediate response and cytotoxicity effect on myocardial cells by extracellular photosensitization reaction varying irradiance. In 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014 (pp. 5316-5319). [6944826] (2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2014.6944826