Immediate response of Ca2+ concentration in myocardial cells against oxidation stress by extracellular photosensitization reaction using Talaporfin sodium for the arrhythmia treatment application

Emiyu Ogawa, Mei Takahashi, Arisa Ito, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We studied the immediate response of myocardial cells by continuous observation using confocal microscope against oxidation stress by extracellular photosensitization reaction using Talaporfin sodium for tachyarrhythmia treatment application. Immediate response in order from several seconds to several minutes is required for the arrhythmia treatment since operators should judge the therapeutic effect during the tachyarrhythmia ablation procedure. To understand the immediate response of myocardial cells, we measured the intracellular Ca2+ concentration using fluo-4 AM during and after the extracellular photosensitization reaction. Talaporfin sodium concentration was varied 10-30 μg/ml. A red diode laser of 663 nm in wavelength was irradiated under the microscope with the radiant exposure of 40 J/cm2 and irradiance of 0.29 W/cm2. We observed the fluorescence image of fluo-4 AM each 400 ms during until 10 min after the photosensitization reaction. The myocardial cell beatings were stopped about 2 s after the beginning of the laser irradiation. The blebs were formed with the Ca2+ inflow. The intracellular Ca2+ was re-decreased after the bleb formation and then the cell necrosis was induced. The cell lethality 10 min after the laser irradiation was less than bleb formation ratio. The time response of the cell necrosis was shortened with the photosensitizer concentration increasing and the minimum average value was 209 s in the case of the 30 μg/ml in photosensitizer concentration and 40 J/cm2 in the radiant exposure. We think this extracellular photosensitization reaction may be applicable to tachyarrhythmia treatment in terms of its immediate response.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume8941
ISBN (Print)9780819498540
DOIs
Publication statusPublished - 2014
EventOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications - San Francisco, CA, United States
Duration: 2014 Feb 22014 Feb 4

Other

OtherOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications
CountryUnited States
CitySan Francisco, CA
Period14/2/214/2/4

Fingerprint

arrhythmia
Photosensitivity Disorders
Photosensitizing Agents
Photosensitizers
Laser beam effects
Cardiac Arrhythmias
Microscopes
Sodium
sodium
Oxidation
oxidation
Blister
Ablation
cells
Tachycardia
Semiconductor lasers
necrosis
Fluorescence
Wavelength
Lasers

Keywords

  • Cardiomyocyte
  • Confocal microscopy
  • Fluo-4 AM
  • NPe6

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Immediate response of Ca2+ concentration in myocardial cells against oxidation stress by extracellular photosensitization reaction using Talaporfin sodium for the arrhythmia treatment application. / Ogawa, Emiyu; Takahashi, Mei; Ito, Arisa; Arai, Tsunenori.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8941 SPIE, 2014. 89410U.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ogawa, E, Takahashi, M, Ito, A & Arai, T 2014, Immediate response of Ca2+ concentration in myocardial cells against oxidation stress by extracellular photosensitization reaction using Talaporfin sodium for the arrhythmia treatment application. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8941, 89410U, SPIE, Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications, San Francisco, CA, United States, 14/2/2. https://doi.org/10.1117/12.2038586
Ogawa, Emiyu ; Takahashi, Mei ; Ito, Arisa ; Arai, Tsunenori. / Immediate response of Ca2+ concentration in myocardial cells against oxidation stress by extracellular photosensitization reaction using Talaporfin sodium for the arrhythmia treatment application. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8941 SPIE, 2014.
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