Immobilization of Natural Products on Glass Slides by Using a Photoaffinity Reaction and the Detection of Protein-Small-Molecule Interactions

Naoki Kanoh, Saori Kumashiro, Siro Simizu, Yasumitsu Kondoh, Satoru Hatakeyama, Hideo Tashiro, Hiroyuki Osada

Research output: Contribution to journalArticlepeer-review

133 Citations (Scopus)

Abstract

Library on a glass slide: A variety of small molecules, including complex natural products, are introduced to glass slides using a photoaffinity reaction (see picture). The small molecules retain the inherent ability to interact with the relevant binding proteins. Importantly, this immobilization procedure does not require a specific functional group, and can be applied for use with a diverse small-molecule library.

Original languageEnglish
Pages (from-to)5584-5587
Number of pages4
JournalAngewandte Chemie - International Edition
Volume42
Issue number45
DOIs
Publication statusPublished - 2003 Nov 24
Externally publishedYes

Keywords

  • High-throughput screening
  • Immobilization
  • Natural products
  • Photochemistry
  • Protein-ligand interactions

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Fingerprint

Dive into the research topics of 'Immobilization of Natural Products on Glass Slides by Using a Photoaffinity Reaction and the Detection of Protein-Small-Molecule Interactions'. Together they form a unique fingerprint.

Cite this