Immobilization of Natural Products on Glass Slides by Using a Photoaffinity Reaction and the Detection of Protein-Small-Molecule Interactions

Naoki Kanoh, Saori Kumashiro, Siro Simizu, Yasumitsu Kondoh, Satoru Hatakeyama, Hideo Tashiro, Hiroyuki Osada

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Library on a glass slide: A variety of small molecules, including complex natural products, are introduced to glass slides using a photoaffinity reaction (see picture). The small molecules retain the inherent ability to interact with the relevant binding proteins. Importantly, this immobilization procedure does not require a specific functional group, and can be applied for use with a diverse small-molecule library.

Original languageEnglish
Pages (from-to)5584-5587
Number of pages4
JournalAngewandte Chemie - International Edition
Volume42
Issue number45
DOIs
Publication statusPublished - 2003 Nov 24
Externally publishedYes

Fingerprint

Biological Products
Proteins
Glass
Molecules
Functional groups
Carrier Proteins

Keywords

  • High-throughput screening
  • Immobilization
  • Natural products
  • Photochemistry
  • Protein-ligand interactions

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Immobilization of Natural Products on Glass Slides by Using a Photoaffinity Reaction and the Detection of Protein-Small-Molecule Interactions. / Kanoh, Naoki; Kumashiro, Saori; Simizu, Siro; Kondoh, Yasumitsu; Hatakeyama, Satoru; Tashiro, Hideo; Osada, Hiroyuki.

In: Angewandte Chemie - International Edition, Vol. 42, No. 45, 24.11.2003, p. 5584-5587.

Research output: Contribution to journalArticle

Kanoh, Naoki ; Kumashiro, Saori ; Simizu, Siro ; Kondoh, Yasumitsu ; Hatakeyama, Satoru ; Tashiro, Hideo ; Osada, Hiroyuki. / Immobilization of Natural Products on Glass Slides by Using a Photoaffinity Reaction and the Detection of Protein-Small-Molecule Interactions. In: Angewandte Chemie - International Edition. 2003 ; Vol. 42, No. 45. pp. 5584-5587.
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