Immobilization of the Gas Signaling Molecule H2S by Radioisotopes: Detection, Quantification, and In Vivo Imaging

Swarbhanu Sarkar, Yeong Su Ha, Nisarg Soni, Gwang Il An, Woonghee Lee, Min Hwan Kim, Phuong Tu Huynh, Heesu Ahn, Nikunj Bhatt, Yong Jin Lee, Jung Young Kim, Kwon Moo Park, Isao Ishii, Shin Geol Kang, Jeongsoo Yoo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Hydrogen sulfide (H2S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H2S in live animals is very challenging. Herein, we report the first radioisotope-based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H2S. Macrocyclic 64Cu complexes that instantly reacted with gaseous H2S to form insoluble 64CuS in a highly sensitive and selective manner were prepared. The H2S concentration in biological samples was measured by a thin-layer radiochromatography method. When 64Cu-cyclen was injected into mice, an elevated H2S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H2S levels in a millimeter-sized infarcted lesion of the rat heart.

Original languageEnglish
JournalAngewandte Chemie - International Edition
DOIs
Publication statusAccepted/In press - 2016

Fingerprint

Radioisotopes
Gases
Imaging techniques
Molecules
Positron emission tomography
Hydrogen Sulfide
Hydrogen sulfide
Luminescence
Rats
Animals

Keywords

  • Gas immobilization
  • Hydrogen sulfide
  • Imaging agents
  • Nuclear imaging
  • Radioisotopes

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Immobilization of the Gas Signaling Molecule H2S by Radioisotopes : Detection, Quantification, and In Vivo Imaging. / Sarkar, Swarbhanu; Ha, Yeong Su; Soni, Nisarg; An, Gwang Il; Lee, Woonghee; Kim, Min Hwan; Huynh, Phuong Tu; Ahn, Heesu; Bhatt, Nikunj; Lee, Yong Jin; Kim, Jung Young; Park, Kwon Moo; Ishii, Isao; Kang, Shin Geol; Yoo, Jeongsoo.

In: Angewandte Chemie - International Edition, 2016.

Research output: Contribution to journalArticle

Sarkar, S, Ha, YS, Soni, N, An, GI, Lee, W, Kim, MH, Huynh, PT, Ahn, H, Bhatt, N, Lee, YJ, Kim, JY, Park, KM, Ishii, I, Kang, SG & Yoo, J 2016, 'Immobilization of the Gas Signaling Molecule H2S by Radioisotopes: Detection, Quantification, and In Vivo Imaging', Angewandte Chemie - International Edition. https://doi.org/10.1002/anie.201603813
Sarkar, Swarbhanu ; Ha, Yeong Su ; Soni, Nisarg ; An, Gwang Il ; Lee, Woonghee ; Kim, Min Hwan ; Huynh, Phuong Tu ; Ahn, Heesu ; Bhatt, Nikunj ; Lee, Yong Jin ; Kim, Jung Young ; Park, Kwon Moo ; Ishii, Isao ; Kang, Shin Geol ; Yoo, Jeongsoo. / Immobilization of the Gas Signaling Molecule H2S by Radioisotopes : Detection, Quantification, and In Vivo Imaging. In: Angewandte Chemie - International Edition. 2016.
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