Immobilization of the Gas Signaling Molecule H2S by Radioisotopes: Detection, Quantification, and In Vivo Imaging

Swarbhanu Sarkar, Yeong Su Ha, Nisarg Soni, Gwang Il An, Woonghee Lee, Min Hwan Kim, Phuong Tu Huynh, Heesu Ahn, Nikunj Bhatt, Yong Jin Lee, Jung Young Kim, Kwon Moo Park, Isao Ishii, Shin Geol Kang, Jeongsoo Yoo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hydrogen sulfide (H2S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H2S in live animals is very challenging. Herein, we report the first radioisotope-based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H2S. Macrocyclic 64Cu complexes that instantly reacted with gaseous H2S to form insoluble 64CuS in a highly sensitive and selective manner were prepared. The H2S concentration in biological samples was measured by a thin-layer radiochromatography method. When 64Cu-cyclen was injected into mice, an elevated H2S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H2S levels in a millimeter-sized infarcted lesion of the rat heart.

Original languageEnglish
JournalAngewandte Chemie - International Edition
DOIs
Publication statusAccepted/In press - 2016

Keywords

  • Gas immobilization
  • Hydrogen sulfide
  • Imaging agents
  • Nuclear imaging
  • Radioisotopes

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

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    Sarkar, S., Ha, Y. S., Soni, N., An, G. I., Lee, W., Kim, M. H., Huynh, P. T., Ahn, H., Bhatt, N., Lee, Y. J., Kim, J. Y., Park, K. M., Ishii, I., Kang, S. G., & Yoo, J. (Accepted/In press). Immobilization of the Gas Signaling Molecule H2S by Radioisotopes: Detection, Quantification, and In Vivo Imaging. Angewandte Chemie - International Edition. https://doi.org/10.1002/anie.201603813