Immortalization of subpopulations of respiratory epithelial cells from transgenic mice bearing SV40 large T antigen

K. Ikeda, J. C. Clark, C. J. Bachurski, K. A. Wikenheiser, J. Cuppoletti, S. Mohanti, R. E. Morris, J. A. Whitsett

Research output: Contribution to journalArticle

Abstract

Murine lung epithelial (MLE) cell lines were produced from lung tumors derived from transgenic mice bearing the viral oncogene, SV40 large T antigen, under transcriptional control of the promoter-enhancer region of the human surfactant protein C (SP-C) gene. Cells were selected on the basis of increased murine cystic fibrosis transmembrane conductance regulator (mCFTR) mRNA content and were dilution cloned to produce distinct immortalized epithelial cell lines. MLE-13a3 cell lines expressing high levels of mCFTR mRNA also expressed apolipoprotein J (apoJ) mRNA, a developmentally regulated glycoprotein expressed preferentially in fetal lung. SP-A, -B, and -C were not detected or were present at low levels in the MLE cells that contained abundant CFTR and apoJ mRNA. In contrast, MLE cells, cloned on the basis of abundant surfactant protein mRNAs, expressed apoJ and mCFTR mRNAs at low levels. Forskolin-stimulated short-circuit current, typical of CFTR-mediated chloride transport activity, was generated by monolayers of subclones of the MLE-13a3 cell lines. Tumor necrosis factor-α stimulated mCFTR mRNA, whereas dexamethasone, retinoic acid, and phorbol ester had no effect on the levels of mCFTR mRNA in MLE-13a3 cells.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume267
Issue number3 11-3
Publication statusPublished - 1994
Externally publishedYes

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Polyomavirus Transforming Antigens
Viral Tumor Antigens
Transgenic Mice
Cystic Fibrosis Transmembrane Conductance Regulator
Epithelial Cells
Lung
Messenger RNA
Clusterin
Cell Line
Surface-Active Agents
Phorbol Esters
Colforsin
Protein C
Tretinoin
Oncogenes
Genetic Promoter Regions
Dexamethasone
Chlorides
Glycoproteins
Tumor Necrosis Factor-alpha

Keywords

  • cystic fibrosis transmembrane conductance regulator
  • lung epithelial cell lines
  • SV40 large T antigen

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Ikeda, K., Clark, J. C., Bachurski, C. J., Wikenheiser, K. A., Cuppoletti, J., Mohanti, S., ... Whitsett, J. A. (1994). Immortalization of subpopulations of respiratory epithelial cells from transgenic mice bearing SV40 large T antigen. American Journal of Physiology - Lung Cellular and Molecular Physiology, 267(3 11-3).

Immortalization of subpopulations of respiratory epithelial cells from transgenic mice bearing SV40 large T antigen. / Ikeda, K.; Clark, J. C.; Bachurski, C. J.; Wikenheiser, K. A.; Cuppoletti, J.; Mohanti, S.; Morris, R. E.; Whitsett, J. A.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 267, No. 3 11-3, 1994.

Research output: Contribution to journalArticle

Ikeda, K, Clark, JC, Bachurski, CJ, Wikenheiser, KA, Cuppoletti, J, Mohanti, S, Morris, RE & Whitsett, JA 1994, 'Immortalization of subpopulations of respiratory epithelial cells from transgenic mice bearing SV40 large T antigen', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 267, no. 3 11-3.
Ikeda, K. ; Clark, J. C. ; Bachurski, C. J. ; Wikenheiser, K. A. ; Cuppoletti, J. ; Mohanti, S. ; Morris, R. E. ; Whitsett, J. A. / Immortalization of subpopulations of respiratory epithelial cells from transgenic mice bearing SV40 large T antigen. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1994 ; Vol. 267, No. 3 11-3.
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