Immotile phenotype of an Escherichia coli mutant lacking the histone- like protein HU

Satoshi Nishida, Tohru Mizushima, Takeyoshi Miki, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The histone-like protein HU in Escherichia coli are encoded by the hup A and hup B genes. A hup A-hup B double deletion mutant has now been shown to express an immotile phenotype. The motility of hup A or hup B single mutants was similar to that of wild-type cells. SDS-polyacrylamide gel electrophoresis revealed that the amount of flagellin in the hup A-hup B double deletion mutant was markedly reduced compared with the wild-type strain, suggesting that the immotile phenotype of the double deletion mutant is caused by a loss of flagella.

Original languageEnglish
Pages (from-to)297-301
Number of pages5
JournalFEMS microbiology letters
Volume150
Issue number2
DOIs
Publication statusPublished - 1997 May 15

Keywords

  • Cell motility
  • Escherichia coli
  • Flagellin
  • HU protein

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Immotile phenotype of an Escherichia coli mutant lacking the histone- like protein HU'. Together they form a unique fingerprint.

  • Cite this