Immotile phenotype of an Escherichia coli mutant lacking the histone- like protein HU

Satoshi Nishida; Tohru Mizushima; Takeyoshi Miki; Kazuhisa Sekimizu

doi:10.1016/S0378-1097(97)00134-1

Immotile phenotype of an Escherichia coli mutant lacking the histone- like protein HU

Satoshi Nishida, Tohru Mizushima, Takeyoshi Miki, Kazuhisa Sekimizu

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The histone-like protein HU in Escherichia coli are encoded by the hup A and hup B genes. A hup A-hup B double deletion mutant has now been shown to express an immotile phenotype. The motility of hup A or hup B single mutants was similar to that of wild-type cells. SDS-polyacrylamide gel electrophoresis revealed that the amount of flagellin in the hup A-hup B double deletion mutant was markedly reduced compared with the wild-type strain, suggesting that the immotile phenotype of the double deletion mutant is caused by a loss of flagella.

Original language	English
Pages (from-to)	297-301
Number of pages	5
Journal	FEMS microbiology letters
Volume	150
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(97)00134-1
Publication status	Published - 1997 May 15
Externally published	Yes

Keywords

Cell motility
Escherichia coli
Flagellin
HU protein

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/S0378-1097(97)00134-1

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title = "Immotile phenotype of an Escherichia coli mutant lacking the histone- like protein HU",

abstract = "The histone-like protein HU in Escherichia coli are encoded by the hup A and hup B genes. A hup A-hup B double deletion mutant has now been shown to express an immotile phenotype. The motility of hup A or hup B single mutants was similar to that of wild-type cells. SDS-polyacrylamide gel electrophoresis revealed that the amount of flagellin in the hup A-hup B double deletion mutant was markedly reduced compared with the wild-type strain, suggesting that the immotile phenotype of the double deletion mutant is caused by a loss of flagella.",

keywords = "Cell motility, Escherichia coli, Flagellin, HU protein",

author = "Satoshi Nishida and Tohru Mizushima and Takeyoshi Miki and Kazuhisa Sekimizu",

note = "Funding Information: We thank Drs. F. Imamoto (Life Technologies Oriental, Tokyo, Japan), K. Kutsukake (Hiroshima University, Hiroshima, Japan) and Y. Kano (Kyoto Pharmaceutical University, Kyoto, Japan) for providing bacterial strains and helpful discussion. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan.",

year = "1997",

month = may,

day = "15",

doi = "10.1016/S0378-1097(97)00134-1",

language = "English",

volume = "150",

pages = "297--301",

journal = "FEMS Microbiology Letters",

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T1 - Immotile phenotype of an Escherichia coli mutant lacking the histone- like protein HU

AU - Nishida, Satoshi

AU - Mizushima, Tohru

AU - Miki, Takeyoshi

AU - Sekimizu, Kazuhisa

N1 - Funding Information: We thank Drs. F. Imamoto (Life Technologies Oriental, Tokyo, Japan), K. Kutsukake (Hiroshima University, Hiroshima, Japan) and Y. Kano (Kyoto Pharmaceutical University, Kyoto, Japan) for providing bacterial strains and helpful discussion. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan.

PY - 1997/5/15

Y1 - 1997/5/15

N2 - The histone-like protein HU in Escherichia coli are encoded by the hup A and hup B genes. A hup A-hup B double deletion mutant has now been shown to express an immotile phenotype. The motility of hup A or hup B single mutants was similar to that of wild-type cells. SDS-polyacrylamide gel electrophoresis revealed that the amount of flagellin in the hup A-hup B double deletion mutant was markedly reduced compared with the wild-type strain, suggesting that the immotile phenotype of the double deletion mutant is caused by a loss of flagella.

AB - The histone-like protein HU in Escherichia coli are encoded by the hup A and hup B genes. A hup A-hup B double deletion mutant has now been shown to express an immotile phenotype. The motility of hup A or hup B single mutants was similar to that of wild-type cells. SDS-polyacrylamide gel electrophoresis revealed that the amount of flagellin in the hup A-hup B double deletion mutant was markedly reduced compared with the wild-type strain, suggesting that the immotile phenotype of the double deletion mutant is caused by a loss of flagella.

KW - Cell motility

KW - Escherichia coli

KW - Flagellin

KW - HU protein

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AN - SCOPUS:0030914036

SN - 0378-1097

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Immotile phenotype of an Escherichia coli mutant lacking the histone- like protein HU

Abstract

Keywords

ASJC Scopus subject areas

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