Immune system expression of SLURP-1 and SLURP-2, two endogenous nicotinic acetylcholine receptor ligands

Yasuhiro Moriwaki, Ken Yoshikawa, Hiromi Fukuda, Yoshihito X. Fujii, Hidemi Misawa, Koichiro Kawashima

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A novel transduction pathway via which apoptosis of keratinocytes is regulated through nicotinic acetylcholine (ACh) receptors (nAChRs) has emerged in studies of secreted mammalian Ly6/urokinase plasminogen-type activator receptor-related protein-1 and-2 (SLURP-1 and SLURP-2, respectively). SLURP-1 reportedly binds to α7 nAChRs and enhances the amplitude of macroscopic currents induced by ACh, leading to facilitation of apoptosis, whereas SLURP-2 binds to α3 nAChRs and prevents apoptosis. These observations prompted us to test whether SLURPs are expressed in immune cells and are involved in the regulation of immune function. We initially used reverse transcription-polymerase chain reaction analysis to characterize the expression profiles of SLURP mRNAs in several murine tissues and organs. Although SLURP-1 mRNA was not expressed in the pancreas, all other tissues and organs tested, including spleen and thymus, expressed both SLURP-1 and SLURP-2 mRNAs. Expression of both mRNAs also was detected in T and B cells, bone marrow-derived dendritic cells (DCs) and macrophages. Moreover, as in keratinocytes, stimulation of MOLT-3 human leukemic T cells with recombinant human SLURP-1 evoked intracellular Ca2+ signaling. These results suggest that both SLURP-1 and SLURP-2 are expressed in various immune cells and organs, and that not only ACh but also SLURPs may be involved in regulating lymphocyte function via nAChR-mediated pathways.

Original languageEnglish
Pages (from-to)2365-2368
Number of pages4
JournalLife Sciences
Volume80
Issue number24-25
DOIs
Publication statusPublished - 2007 May 30

Keywords

  • Dendritic cells
  • Lymphocytes
  • Macrophages
  • Nicotinic acetylcholine receptor
  • SLURP-1
  • SLURP-2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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