Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries

Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano, Mikiko C. Siomi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specifi c proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specifi c target labeled with both fl uorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

Original languageEnglish
Pages (from-to)163-178
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume1328
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Electron microscopic in situ hybridization
  • Electron microscopy
  • EM-ISH
  • Flam
  • Fluorescence immunohistochemistry
  • Immuno-electron microscopy
  • In situ hybridization
  • ISH
  • PiRNAs
  • PIWI-interacting RNAs
  • Yb
  • Zuc

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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