Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries

Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano, Mikiko C. Siomi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

Original languageEnglish
Title of host publicationDrosophila Oogenesis
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages163-178
Number of pages16
ISBN (Electronic)9781493928514
ISBN (Print)9781493928507
DOIs
Publication statusPublished - 2015 Aug 31

Keywords

  • EM-ISH
  • Electron microscopic in situ hybridization
  • Electron microscopy
  • Flam
  • Fluorescence immunohistochemistry
  • Immuno-electron microscopy
  • In situ hybridization
  • Ish
  • PIWI-interacting RNAs
  • Yb
  • Zuc
  • piRNAs

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Medicine(all)

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    Shibata, S., Murota, Y., Nishimoto, Y., Yoshimura, M., Nagai, T., Okano, H., & Siomi, M. C. (2015). Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries. In Drosophila Oogenesis: Methods and Protocols (pp. 163-178). Springer New York. https://doi.org/10.1007/978-1-4939-2851-4_12