Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries

Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano, Mikiko C. Siomi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

Original languageEnglish
Title of host publicationDrosophila Oogenesis: Methods and Protocols
PublisherSpringer New York
Pages163-178
Number of pages16
ISBN (Print)9781493928514, 9781493928507
DOIs
Publication statusPublished - 2015 Aug 31

Fingerprint

Immunoelectron Microscopy
somatic cells
Gold
in situ hybridization
gold
Primates
Electron microscopy
Drosophila
In Situ Hybridization
Ovary
Rodentia
electron microscopy
rodents
Fluorescence
electrons
fluorescence
RNA
Electrons
Proteins
proteins

Keywords

  • Electron microscopic in situ hybridization
  • Electron microscopy
  • EM-ISH
  • Flam
  • Fluorescence immunohistochemistry
  • Immuno-electron microscopy
  • In situ hybridization
  • Ish
  • piRNAs
  • PIWI-interacting RNAs
  • Yb
  • Zuc

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Shibata, S., Murota, Y., Nishimoto, Y., Yoshimura, M., Nagai, T., Okano, H., & Siomi, M. C. (2015). Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries. In Drosophila Oogenesis: Methods and Protocols (pp. 163-178). Springer New York. https://doi.org/10.1007/978-1-4939-2851-4_12

Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries. / Shibata, Shinsuke; Murota, Yukiko; Nishimoto, Yoshinori; Yoshimura, Mana; Nagai, Toshihiro; Okano, Hideyuki; Siomi, Mikiko C.

Drosophila Oogenesis: Methods and Protocols. Springer New York, 2015. p. 163-178.

Research output: Chapter in Book/Report/Conference proceedingChapter

Shibata, S, Murota, Y, Nishimoto, Y, Yoshimura, M, Nagai, T, Okano, H & Siomi, MC 2015, Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries. in Drosophila Oogenesis: Methods and Protocols. Springer New York, pp. 163-178. https://doi.org/10.1007/978-1-4939-2851-4_12
Shibata S, Murota Y, Nishimoto Y, Yoshimura M, Nagai T, Okano H et al. Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries. In Drosophila Oogenesis: Methods and Protocols. Springer New York. 2015. p. 163-178 https://doi.org/10.1007/978-1-4939-2851-4_12
Shibata, Shinsuke ; Murota, Yukiko ; Nishimoto, Yoshinori ; Yoshimura, Mana ; Nagai, Toshihiro ; Okano, Hideyuki ; Siomi, Mikiko C. / Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries. Drosophila Oogenesis: Methods and Protocols. Springer New York, 2015. pp. 163-178
@inbook{f0d72c2b209a45168b6dc2bd2a99be45,
title = "Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries",
abstract = "Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.",
keywords = "Electron microscopic in situ hybridization, Electron microscopy, EM-ISH, Flam, Fluorescence immunohistochemistry, Immuno-electron microscopy, In situ hybridization, Ish, piRNAs, PIWI-interacting RNAs, Yb, Zuc",
author = "Shinsuke Shibata and Yukiko Murota and Yoshinori Nishimoto and Mana Yoshimura and Toshihiro Nagai and Hideyuki Okano and Siomi, {Mikiko C.}",
year = "2015",
month = "8",
day = "31",
doi = "10.1007/978-1-4939-2851-4_12",
language = "English",
isbn = "9781493928514",
pages = "163--178",
booktitle = "Drosophila Oogenesis: Methods and Protocols",
publisher = "Springer New York",

}

TY - CHAP

T1 - Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries

AU - Shibata, Shinsuke

AU - Murota, Yukiko

AU - Nishimoto, Yoshinori

AU - Yoshimura, Mana

AU - Nagai, Toshihiro

AU - Okano, Hideyuki

AU - Siomi, Mikiko C.

PY - 2015/8/31

Y1 - 2015/8/31

N2 - Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

AB - Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

KW - Electron microscopic in situ hybridization

KW - Electron microscopy

KW - EM-ISH

KW - Flam

KW - Fluorescence immunohistochemistry

KW - Immuno-electron microscopy

KW - In situ hybridization

KW - Ish

KW - piRNAs

KW - PIWI-interacting RNAs

KW - Yb

KW - Zuc

UR - http://www.scopus.com/inward/record.url?scp=84955125740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84955125740&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-2851-4_12

DO - 10.1007/978-1-4939-2851-4_12

M3 - Chapter

C2 - 26324437

AN - SCOPUS:84955125740

SN - 9781493928514

SN - 9781493928507

SP - 163

EP - 178

BT - Drosophila Oogenesis: Methods and Protocols

PB - Springer New York

ER -