Immunodeficient NOD-scid IL-2Rγnull mice do not display T and B cell leakiness

Ikumi Katano, Ryoji ITO, Tomoo Eto, Sadakazu Aiso, Mamoru Ito

Research output: Contribution to journalComment/debate

14 Citations (Scopus)

Abstract

NOD/Shi-scid IL-2Rγnull (NOG) mice established by introducing the IL-2Rγnull gene of IL-2Rγ KO mice into NOD/Shi-scid mice by backcross-mating show a high xenograft engraftment level and are therefore well suited as a humanized mouse model. SCID mice bearing the Prkdcscid gene show a high incidence of thymic lymphoma and a leaky phenomenon in which a few clonal T and B cells develop in aged mice. In the present study, NOG mice were assessed for the presence of a leaky phenomenon such as the one observed in C.B-17-scid and NOD-scid mice. Serum immunoglobulin analysis did not detect IgG or IgM in NOG mice, unlike the findings in C.B-17-scid and NOD-scid mice. Flow cytometry analysis revealed the absence of T and B cells in the peripheral blood and spleens of NOG mice. These results reflect the suppression of the leaky phenomenon in NOG mice through the inactivation of the IL-2Rγ gene, which is commonly expressed in T and B cell growth factor receptors to IL-2, IL-4 and IL-7.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalExperimental Animals
Volume60
Issue number2
DOIs
Publication statusPublished - 2011 May 3

Keywords

  • Immunoglobulin
  • Leakiness
  • NOD-scid il-2Rγnull mice

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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