Immunoglobulin heavy chain variable region(VH) genes in B cell clones producing anti-colon antibodies in ulcerative colitis

Nagamu Inoue, T. Hisamatsu, Y. Iwao, M. Watanabe, H. Ishii, Y. Mizuno, T. Hibi

Research output: Contribution to journalReview article

Abstract

We have established anti-colon antibodies producing B cell clones from the patient with ulcerative colitis, and analyzed the immunoglobulin heavy chain variable region (VH) gene segments of these clones using RT-PCR with VH family specific primers. VH3 family was used by all ten clones from ulcerative colitis that produced anti-colon antibodies, while various VH gene families were used by eight clones from normal controls. By nucleotide sequence analysis, two LPL clones were thought to be derived from a germ line gene segment, VH26. However, other three PBL clones were derived from 22-2B, 9-1 and 1.9-III, respectively. These results suggest the heterogeneity of the colonic antigens that autoantibodies in ulcerative colitis recognize.

Original languageEnglish
Pages (from-to)1176-1181
Number of pages6
JournalNippon rinsho. Japanese journal of clinical medicine
Volume54
Issue number4
Publication statusPublished - 1996 Apr 1
Externally publishedYes

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Immunoglobulin Heavy Chains
Ulcerative Colitis
Anti-Idiotypic Antibodies
Colon
B-Lymphocytes
Clone Cells
Genes
Antibody-Producing Cells
Germ Cells
Autoantibodies
Sequence Analysis
Antigens
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

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Immunoglobulin heavy chain variable region(VH) genes in B cell clones producing anti-colon antibodies in ulcerative colitis. / Inoue, Nagamu; Hisamatsu, T.; Iwao, Y.; Watanabe, M.; Ishii, H.; Mizuno, Y.; Hibi, T.

In: Nippon rinsho. Japanese journal of clinical medicine, Vol. 54, No. 4, 01.04.1996, p. 1176-1181.

Research output: Contribution to journalReview article

Inoue, Nagamu ; Hisamatsu, T. ; Iwao, Y. ; Watanabe, M. ; Ishii, H. ; Mizuno, Y. ; Hibi, T. / Immunoglobulin heavy chain variable region(VH) genes in B cell clones producing anti-colon antibodies in ulcerative colitis. In: Nippon rinsho. Japanese journal of clinical medicine. 1996 ; Vol. 54, No. 4. pp. 1176-1181.
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