Immunohistochemical and genetic characteristics of lung cancer mimicking organizing pneumonia

Tomohiro Ichikawa, Koichi Saruwatari, Sachiyo Mimaki, Masato Sugano, Keiju Aokage, Motohiro Kojima, Tomoyuki Hishida, Satoshi Fujii, Junji Yoshida, Takeshi Kuwata, Atsushi Ochiai, Kenji Suzuki, Masahiro Tsuboi, Koichi Goto, Katsuya Tsuchihara, Genichiro Ishii

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Introduction Lung cancer mimicking organizing pneumonia (LCOP) is a novel radiological entity of lung adenocarcinoma that could be misdiagnosed as inflammatory lesions. However, the characteristic biological and genetic features of LCOP are not fully clarified. Materials and methods We used thin-section CT images to select cases of (LCOP) among surgically resected lung adenocarcinoma patients. We compared the clinicopathological characteristics and the immunophenotypes of LCOP (n = 44) and other lepidic-predominant adenocarcinomas (non-LCOP, n = 56). We also analyzed the genomic mutation features of LCOP (n = 4) by whole-exome sequencing (WES). Results All LCOP lesions were lepidic-predominant invasive adenocarcinoma. Patients with LCOP had significantly superior recurrence-free survival, compared to non-LCOP patients (95.5% and 74.4%; P = 0.006, respectively). Vascular invasion and lymph node metastasis were less frequent in LCOP than in non-LCOP patients (P = 0.001 and P = 0.03, respectively). The cancer cell expression levels of aggressiveness-related molecules, including ezrin, ALDH-1, laminin-5 were similar between LCOP and non-LCOP. On the contrary, the number of tumor promoting stromal cells, i.e., podoplanin-positive cancer-associated fibroblasts and CD204-positive tumor associated macrophages, was significantly lower in LOCP (P = 0.021 and P = 0.037, respectively). WES revealed that ABCB1, DNAH3, MSI2, and SLITRK2 were specifically mutated in LCOP. Conclusions Our results indicate that LCOP is characterized by fewer tumor-promoting stromal cells, which may contribute to the better prognosis of LCOP patients. Moreover, recognition of specific somatic mutations of LCOP patients may provide information regarding the development and progression of this type of lung cancer.

Original languageEnglish
Pages (from-to)134-139
Number of pages6
JournalLung Cancer
Volume113
DOIs
Publication statusPublished - 2017 Nov 1
Externally publishedYes

Fingerprint

Lung Neoplasms
Pneumonia
Neoplasms
Exome
Stromal Cells
Mutation
Diagnostic Errors
Blood Vessels
Adenocarcinoma
Lymph Nodes
Macrophages

Keywords

  • Lung cancer
  • Microenvironment
  • Stromal cell
  • Whole exome sequence

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research

Cite this

Ichikawa, T., Saruwatari, K., Mimaki, S., Sugano, M., Aokage, K., Kojima, M., ... Ishii, G. (2017). Immunohistochemical and genetic characteristics of lung cancer mimicking organizing pneumonia. Lung Cancer, 113, 134-139. https://doi.org/10.1016/j.lungcan.2017.10.001

Immunohistochemical and genetic characteristics of lung cancer mimicking organizing pneumonia. / Ichikawa, Tomohiro; Saruwatari, Koichi; Mimaki, Sachiyo; Sugano, Masato; Aokage, Keiju; Kojima, Motohiro; Hishida, Tomoyuki; Fujii, Satoshi; Yoshida, Junji; Kuwata, Takeshi; Ochiai, Atsushi; Suzuki, Kenji; Tsuboi, Masahiro; Goto, Koichi; Tsuchihara, Katsuya; Ishii, Genichiro.

In: Lung Cancer, Vol. 113, 01.11.2017, p. 134-139.

Research output: Contribution to journalArticle

Ichikawa, T, Saruwatari, K, Mimaki, S, Sugano, M, Aokage, K, Kojima, M, Hishida, T, Fujii, S, Yoshida, J, Kuwata, T, Ochiai, A, Suzuki, K, Tsuboi, M, Goto, K, Tsuchihara, K & Ishii, G 2017, 'Immunohistochemical and genetic characteristics of lung cancer mimicking organizing pneumonia', Lung Cancer, vol. 113, pp. 134-139. https://doi.org/10.1016/j.lungcan.2017.10.001
Ichikawa T, Saruwatari K, Mimaki S, Sugano M, Aokage K, Kojima M et al. Immunohistochemical and genetic characteristics of lung cancer mimicking organizing pneumonia. Lung Cancer. 2017 Nov 1;113:134-139. https://doi.org/10.1016/j.lungcan.2017.10.001
Ichikawa, Tomohiro ; Saruwatari, Koichi ; Mimaki, Sachiyo ; Sugano, Masato ; Aokage, Keiju ; Kojima, Motohiro ; Hishida, Tomoyuki ; Fujii, Satoshi ; Yoshida, Junji ; Kuwata, Takeshi ; Ochiai, Atsushi ; Suzuki, Kenji ; Tsuboi, Masahiro ; Goto, Koichi ; Tsuchihara, Katsuya ; Ishii, Genichiro. / Immunohistochemical and genetic characteristics of lung cancer mimicking organizing pneumonia. In: Lung Cancer. 2017 ; Vol. 113. pp. 134-139.
@article{5c14d9b1a2e744daa0c5f5586e7d79c8,
title = "Immunohistochemical and genetic characteristics of lung cancer mimicking organizing pneumonia",
abstract = "Introduction Lung cancer mimicking organizing pneumonia (LCOP) is a novel radiological entity of lung adenocarcinoma that could be misdiagnosed as inflammatory lesions. However, the characteristic biological and genetic features of LCOP are not fully clarified. Materials and methods We used thin-section CT images to select cases of (LCOP) among surgically resected lung adenocarcinoma patients. We compared the clinicopathological characteristics and the immunophenotypes of LCOP (n = 44) and other lepidic-predominant adenocarcinomas (non-LCOP, n = 56). We also analyzed the genomic mutation features of LCOP (n = 4) by whole-exome sequencing (WES). Results All LCOP lesions were lepidic-predominant invasive adenocarcinoma. Patients with LCOP had significantly superior recurrence-free survival, compared to non-LCOP patients (95.5{\%} and 74.4{\%}; P = 0.006, respectively). Vascular invasion and lymph node metastasis were less frequent in LCOP than in non-LCOP patients (P = 0.001 and P = 0.03, respectively). The cancer cell expression levels of aggressiveness-related molecules, including ezrin, ALDH-1, laminin-5 were similar between LCOP and non-LCOP. On the contrary, the number of tumor promoting stromal cells, i.e., podoplanin-positive cancer-associated fibroblasts and CD204-positive tumor associated macrophages, was significantly lower in LOCP (P = 0.021 and P = 0.037, respectively). WES revealed that ABCB1, DNAH3, MSI2, and SLITRK2 were specifically mutated in LCOP. Conclusions Our results indicate that LCOP is characterized by fewer tumor-promoting stromal cells, which may contribute to the better prognosis of LCOP patients. Moreover, recognition of specific somatic mutations of LCOP patients may provide information regarding the development and progression of this type of lung cancer.",
keywords = "Lung cancer, Microenvironment, Stromal cell, Whole exome sequence",
author = "Tomohiro Ichikawa and Koichi Saruwatari and Sachiyo Mimaki and Masato Sugano and Keiju Aokage and Motohiro Kojima and Tomoyuki Hishida and Satoshi Fujii and Junji Yoshida and Takeshi Kuwata and Atsushi Ochiai and Kenji Suzuki and Masahiro Tsuboi and Koichi Goto and Katsuya Tsuchihara and Genichiro Ishii",
year = "2017",
month = "11",
day = "1",
doi = "10.1016/j.lungcan.2017.10.001",
language = "English",
volume = "113",
pages = "134--139",
journal = "Lung Cancer",
issn = "0169-5002",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Immunohistochemical and genetic characteristics of lung cancer mimicking organizing pneumonia

AU - Ichikawa, Tomohiro

AU - Saruwatari, Koichi

AU - Mimaki, Sachiyo

AU - Sugano, Masato

AU - Aokage, Keiju

AU - Kojima, Motohiro

AU - Hishida, Tomoyuki

AU - Fujii, Satoshi

AU - Yoshida, Junji

AU - Kuwata, Takeshi

AU - Ochiai, Atsushi

AU - Suzuki, Kenji

AU - Tsuboi, Masahiro

AU - Goto, Koichi

AU - Tsuchihara, Katsuya

AU - Ishii, Genichiro

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Introduction Lung cancer mimicking organizing pneumonia (LCOP) is a novel radiological entity of lung adenocarcinoma that could be misdiagnosed as inflammatory lesions. However, the characteristic biological and genetic features of LCOP are not fully clarified. Materials and methods We used thin-section CT images to select cases of (LCOP) among surgically resected lung adenocarcinoma patients. We compared the clinicopathological characteristics and the immunophenotypes of LCOP (n = 44) and other lepidic-predominant adenocarcinomas (non-LCOP, n = 56). We also analyzed the genomic mutation features of LCOP (n = 4) by whole-exome sequencing (WES). Results All LCOP lesions were lepidic-predominant invasive adenocarcinoma. Patients with LCOP had significantly superior recurrence-free survival, compared to non-LCOP patients (95.5% and 74.4%; P = 0.006, respectively). Vascular invasion and lymph node metastasis were less frequent in LCOP than in non-LCOP patients (P = 0.001 and P = 0.03, respectively). The cancer cell expression levels of aggressiveness-related molecules, including ezrin, ALDH-1, laminin-5 were similar between LCOP and non-LCOP. On the contrary, the number of tumor promoting stromal cells, i.e., podoplanin-positive cancer-associated fibroblasts and CD204-positive tumor associated macrophages, was significantly lower in LOCP (P = 0.021 and P = 0.037, respectively). WES revealed that ABCB1, DNAH3, MSI2, and SLITRK2 were specifically mutated in LCOP. Conclusions Our results indicate that LCOP is characterized by fewer tumor-promoting stromal cells, which may contribute to the better prognosis of LCOP patients. Moreover, recognition of specific somatic mutations of LCOP patients may provide information regarding the development and progression of this type of lung cancer.

AB - Introduction Lung cancer mimicking organizing pneumonia (LCOP) is a novel radiological entity of lung adenocarcinoma that could be misdiagnosed as inflammatory lesions. However, the characteristic biological and genetic features of LCOP are not fully clarified. Materials and methods We used thin-section CT images to select cases of (LCOP) among surgically resected lung adenocarcinoma patients. We compared the clinicopathological characteristics and the immunophenotypes of LCOP (n = 44) and other lepidic-predominant adenocarcinomas (non-LCOP, n = 56). We also analyzed the genomic mutation features of LCOP (n = 4) by whole-exome sequencing (WES). Results All LCOP lesions were lepidic-predominant invasive adenocarcinoma. Patients with LCOP had significantly superior recurrence-free survival, compared to non-LCOP patients (95.5% and 74.4%; P = 0.006, respectively). Vascular invasion and lymph node metastasis were less frequent in LCOP than in non-LCOP patients (P = 0.001 and P = 0.03, respectively). The cancer cell expression levels of aggressiveness-related molecules, including ezrin, ALDH-1, laminin-5 were similar between LCOP and non-LCOP. On the contrary, the number of tumor promoting stromal cells, i.e., podoplanin-positive cancer-associated fibroblasts and CD204-positive tumor associated macrophages, was significantly lower in LOCP (P = 0.021 and P = 0.037, respectively). WES revealed that ABCB1, DNAH3, MSI2, and SLITRK2 were specifically mutated in LCOP. Conclusions Our results indicate that LCOP is characterized by fewer tumor-promoting stromal cells, which may contribute to the better prognosis of LCOP patients. Moreover, recognition of specific somatic mutations of LCOP patients may provide information regarding the development and progression of this type of lung cancer.

KW - Lung cancer

KW - Microenvironment

KW - Stromal cell

KW - Whole exome sequence

UR - http://www.scopus.com/inward/record.url?scp=85030770832&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030770832&partnerID=8YFLogxK

U2 - 10.1016/j.lungcan.2017.10.001

DO - 10.1016/j.lungcan.2017.10.001

M3 - Article

C2 - 29110840

AN - SCOPUS:85030770832

VL - 113

SP - 134

EP - 139

JO - Lung Cancer

JF - Lung Cancer

SN - 0169-5002

ER -