Immunohistochemical changes in production of pituitary hormones during artificial maturation of female Japanese eel Anguilla japonica

Yuichi Ozaki, Koichi Ishida, Koji Saito, Kazuhiro Ura, Shinji Adachi, Kohei Yamauchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Specific antibodies against follicle-stimulating hormone β subunit (FSHβ), prolactin (PRL), and somatolactin (SL) of the Japanese eel Anguilla japonica were produced. These antibodies, as well as antibodies against luteinizing hormone β subunit (LHβ) and growth hormone (GH) produced previously, were used to examine changes in the production of pituitary hormones in female eels during maturation induced by salmon pituitary homogenate (SPH) injection. Immunohistochemical observations showed a decrease in FSH production after SPH injection, suggesting that SPH inhibits FSH production. In contrast, LH production increased markedly with maturation. The number of GH producing cells decreased gradually during maturation, possibly because of inhibition by exogenous GH present in the SPH and/or endogenous insulin-like growth factor-I produced by the stimulation of salmon GH. Although changes in the number of PRL producing cells with maturation were not evident, the number of SL producing cells showed a peak at the late vitellogenic stages, and thereafter decreased to the migratory nucleus stage. These results suggest that GH and SL are involved in sexual maturation in SPH injected eels, as in other fishes.

Original languageEnglish
Pages (from-to)574-584
Number of pages11
JournalFisheries Science
Volume73
Issue number3
DOIs
Publication statusPublished - 2007 Jun
Externally publishedYes

Fingerprint

pituitary hormones
Anguilla japonica
eel
hormone
salmon
maturation
somatotropin
follicle-stimulating hormone
luteinizing hormone
prolactin
antibodies
antibody
injection
cells
insulin-like growth factor I
fish

Keywords

  • Gonadotropin
  • Growth hormone
  • Immunohistochemistry
  • Japanese eel
  • Maturation
  • Prolactin
  • Somatolactin

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Immunohistochemical changes in production of pituitary hormones during artificial maturation of female Japanese eel Anguilla japonica. / Ozaki, Yuichi; Ishida, Koichi; Saito, Koji; Ura, Kazuhiro; Adachi, Shinji; Yamauchi, Kohei.

In: Fisheries Science, Vol. 73, No. 3, 06.2007, p. 574-584.

Research output: Contribution to journalArticle

Ozaki, Yuichi ; Ishida, Koichi ; Saito, Koji ; Ura, Kazuhiro ; Adachi, Shinji ; Yamauchi, Kohei. / Immunohistochemical changes in production of pituitary hormones during artificial maturation of female Japanese eel Anguilla japonica. In: Fisheries Science. 2007 ; Vol. 73, No. 3. pp. 574-584.
@article{a6f4213a1ea343b4a65167e16d61e706,
title = "Immunohistochemical changes in production of pituitary hormones during artificial maturation of female Japanese eel Anguilla japonica",
abstract = "Specific antibodies against follicle-stimulating hormone β subunit (FSHβ), prolactin (PRL), and somatolactin (SL) of the Japanese eel Anguilla japonica were produced. These antibodies, as well as antibodies against luteinizing hormone β subunit (LHβ) and growth hormone (GH) produced previously, were used to examine changes in the production of pituitary hormones in female eels during maturation induced by salmon pituitary homogenate (SPH) injection. Immunohistochemical observations showed a decrease in FSH production after SPH injection, suggesting that SPH inhibits FSH production. In contrast, LH production increased markedly with maturation. The number of GH producing cells decreased gradually during maturation, possibly because of inhibition by exogenous GH present in the SPH and/or endogenous insulin-like growth factor-I produced by the stimulation of salmon GH. Although changes in the number of PRL producing cells with maturation were not evident, the number of SL producing cells showed a peak at the late vitellogenic stages, and thereafter decreased to the migratory nucleus stage. These results suggest that GH and SL are involved in sexual maturation in SPH injected eels, as in other fishes.",
keywords = "Gonadotropin, Growth hormone, Immunohistochemistry, Japanese eel, Maturation, Prolactin, Somatolactin",
author = "Yuichi Ozaki and Koichi Ishida and Koji Saito and Kazuhiro Ura and Shinji Adachi and Kohei Yamauchi",
year = "2007",
month = "6",
doi = "10.1111/j.1444-2906.2007.01370.x",
language = "English",
volume = "73",
pages = "574--584",
journal = "Fisheries Science",
issn = "0919-9268",
publisher = "Springer Japan",
number = "3",

}

TY - JOUR

T1 - Immunohistochemical changes in production of pituitary hormones during artificial maturation of female Japanese eel Anguilla japonica

AU - Ozaki, Yuichi

AU - Ishida, Koichi

AU - Saito, Koji

AU - Ura, Kazuhiro

AU - Adachi, Shinji

AU - Yamauchi, Kohei

PY - 2007/6

Y1 - 2007/6

N2 - Specific antibodies against follicle-stimulating hormone β subunit (FSHβ), prolactin (PRL), and somatolactin (SL) of the Japanese eel Anguilla japonica were produced. These antibodies, as well as antibodies against luteinizing hormone β subunit (LHβ) and growth hormone (GH) produced previously, were used to examine changes in the production of pituitary hormones in female eels during maturation induced by salmon pituitary homogenate (SPH) injection. Immunohistochemical observations showed a decrease in FSH production after SPH injection, suggesting that SPH inhibits FSH production. In contrast, LH production increased markedly with maturation. The number of GH producing cells decreased gradually during maturation, possibly because of inhibition by exogenous GH present in the SPH and/or endogenous insulin-like growth factor-I produced by the stimulation of salmon GH. Although changes in the number of PRL producing cells with maturation were not evident, the number of SL producing cells showed a peak at the late vitellogenic stages, and thereafter decreased to the migratory nucleus stage. These results suggest that GH and SL are involved in sexual maturation in SPH injected eels, as in other fishes.

AB - Specific antibodies against follicle-stimulating hormone β subunit (FSHβ), prolactin (PRL), and somatolactin (SL) of the Japanese eel Anguilla japonica were produced. These antibodies, as well as antibodies against luteinizing hormone β subunit (LHβ) and growth hormone (GH) produced previously, were used to examine changes in the production of pituitary hormones in female eels during maturation induced by salmon pituitary homogenate (SPH) injection. Immunohistochemical observations showed a decrease in FSH production after SPH injection, suggesting that SPH inhibits FSH production. In contrast, LH production increased markedly with maturation. The number of GH producing cells decreased gradually during maturation, possibly because of inhibition by exogenous GH present in the SPH and/or endogenous insulin-like growth factor-I produced by the stimulation of salmon GH. Although changes in the number of PRL producing cells with maturation were not evident, the number of SL producing cells showed a peak at the late vitellogenic stages, and thereafter decreased to the migratory nucleus stage. These results suggest that GH and SL are involved in sexual maturation in SPH injected eels, as in other fishes.

KW - Gonadotropin

KW - Growth hormone

KW - Immunohistochemistry

KW - Japanese eel

KW - Maturation

KW - Prolactin

KW - Somatolactin

UR - http://www.scopus.com/inward/record.url?scp=34250205273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250205273&partnerID=8YFLogxK

U2 - 10.1111/j.1444-2906.2007.01370.x

DO - 10.1111/j.1444-2906.2007.01370.x

M3 - Article

AN - SCOPUS:34250205273

VL - 73

SP - 574

EP - 584

JO - Fisheries Science

JF - Fisheries Science

SN - 0919-9268

IS - 3

ER -