Immunohistochemical detection of GLUT-1 can discriminate between reactive mesothelium and malignant mesothelioma

Yasufumi Kato, Koji Tsuta, Kunihiko Seki, Akiko Miyagi Maeshima, Shunichi Watanabe, Kenji Suzuki, Hisao Asamura, Ryosuke Tsuchiya, Yoshihiro Matsuno

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

The separation of benign reactive mesothelium (RM) from malignant mesothelial proliferation can be a major challenge. A number of markers have been proposed, including epithelial membrane antigen, p53 protein, and P-glycoprotein. To date, however, no immunohistochemical marker that allows unequivocal discrimination of RM from malignant pleural mesothelioma (MPM) has been available. A family of glucose transporter isoforms (GLUT), of which GLUT-1 is a member, facilitate the entry of glucose into cells. GLUT-1 is largely undetectable by immunohistochemistry in normal epithelial tissues and benign tumors, but is expressed in a variety of malignancies. Thus, the expression of GLUT-1 appears to be a potential marker of malignant transformation. Recently, in fact, some studies have shown that GLUT-1 expression is useful for distinguishing benign from malignant lesions. The purpose of the present study was to evaluate the diagnostic utility of GLUT-1 expression for diagnostic differentiation between RM and MPM. Immunohistochemical staining for GLUT-1 was performed in 40 cases of RM, 48 cases of MPM, and 58 cases of lung carcinoma. Immunohistochemical GLUT-1 expression was seen in 40 of 40 (100%) MPMs, and in all cases the expression was demonstrated by linear plasma membrane staining, sometimes with cytoplasmic staining in addition. GLUT-1 expression was also observed in 56 out of 58 (96.5%) lung carcinomas. On the other hand, no RM cases were positive for GLUT-1. GLUT-1 is a sensitive and specific immunohistochemical marker enabling differential diagnosis of RM from MPM, whereas it cannot discriminate MPM from lung carcinoma.

Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalModern Pathology
Volume20
Issue number2
DOIs
Publication statusPublished - 2007 Feb
Externally publishedYes

Fingerprint

Facilitative Glucose Transport Proteins
Protein Isoforms
Epithelium
Staining and Labeling
Carcinoma
Lung
Malignant Mesothelioma
Mucin-1
P-Glycoprotein
Neoplasms
Differential Diagnosis
Immunohistochemistry
Cell Membrane
Glucose

Keywords

  • Glut-1
  • Immunohistochemistry
  • Lung carcinoma
  • Malignant pleural mesothelioma
  • Reactive methothelium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Kato, Y., Tsuta, K., Seki, K., Maeshima, A. M., Watanabe, S., Suzuki, K., ... Matsuno, Y. (2007). Immunohistochemical detection of GLUT-1 can discriminate between reactive mesothelium and malignant mesothelioma. Modern Pathology, 20(2), 215-220. https://doi.org/10.1038/modpathol.3800732

Immunohistochemical detection of GLUT-1 can discriminate between reactive mesothelium and malignant mesothelioma. / Kato, Yasufumi; Tsuta, Koji; Seki, Kunihiko; Maeshima, Akiko Miyagi; Watanabe, Shunichi; Suzuki, Kenji; Asamura, Hisao; Tsuchiya, Ryosuke; Matsuno, Yoshihiro.

In: Modern Pathology, Vol. 20, No. 2, 02.2007, p. 215-220.

Research output: Contribution to journalArticle

Kato, Y, Tsuta, K, Seki, K, Maeshima, AM, Watanabe, S, Suzuki, K, Asamura, H, Tsuchiya, R & Matsuno, Y 2007, 'Immunohistochemical detection of GLUT-1 can discriminate between reactive mesothelium and malignant mesothelioma', Modern Pathology, vol. 20, no. 2, pp. 215-220. https://doi.org/10.1038/modpathol.3800732
Kato, Yasufumi ; Tsuta, Koji ; Seki, Kunihiko ; Maeshima, Akiko Miyagi ; Watanabe, Shunichi ; Suzuki, Kenji ; Asamura, Hisao ; Tsuchiya, Ryosuke ; Matsuno, Yoshihiro. / Immunohistochemical detection of GLUT-1 can discriminate between reactive mesothelium and malignant mesothelioma. In: Modern Pathology. 2007 ; Vol. 20, No. 2. pp. 215-220.
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abstract = "The separation of benign reactive mesothelium (RM) from malignant mesothelial proliferation can be a major challenge. A number of markers have been proposed, including epithelial membrane antigen, p53 protein, and P-glycoprotein. To date, however, no immunohistochemical marker that allows unequivocal discrimination of RM from malignant pleural mesothelioma (MPM) has been available. A family of glucose transporter isoforms (GLUT), of which GLUT-1 is a member, facilitate the entry of glucose into cells. GLUT-1 is largely undetectable by immunohistochemistry in normal epithelial tissues and benign tumors, but is expressed in a variety of malignancies. Thus, the expression of GLUT-1 appears to be a potential marker of malignant transformation. Recently, in fact, some studies have shown that GLUT-1 expression is useful for distinguishing benign from malignant lesions. The purpose of the present study was to evaluate the diagnostic utility of GLUT-1 expression for diagnostic differentiation between RM and MPM. Immunohistochemical staining for GLUT-1 was performed in 40 cases of RM, 48 cases of MPM, and 58 cases of lung carcinoma. Immunohistochemical GLUT-1 expression was seen in 40 of 40 (100{\%}) MPMs, and in all cases the expression was demonstrated by linear plasma membrane staining, sometimes with cytoplasmic staining in addition. GLUT-1 expression was also observed in 56 out of 58 (96.5{\%}) lung carcinomas. On the other hand, no RM cases were positive for GLUT-1. GLUT-1 is a sensitive and specific immunohistochemical marker enabling differential diagnosis of RM from MPM, whereas it cannot discriminate MPM from lung carcinoma.",
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