Immunohistochemical detection of K-sam protein in stomach cancer

Yutaka Hattori, Hiroshi Itoh, Shinya Uchino, Kouichi Hosokawa, Atsushi Ochiai, Yoshinori Ino, Hideshi Ishii, Hiromi Sakamoto, Naohito Yamaguchi, Kazuyoshi Yanagihara, Setsuo Hirohashi, Takashi Sugimura, Masaaki Terada

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

The K-sam gene, originally isolated as an amplified gene from the stomach cancer cell line KATO-III, is characterized by its preferential amplification in the undifferentiated type (diffuse type) of stomach cancer and encodes one of the receptors for heparin-binding growth factors or fibroblast growth factors. The K-sam gene has been isolated by different methods and has been designated BEK, TK14, and Cek2. The receptor for keratinocyte growth factor was also found to be encoded by the same gene. To examine the expression of the K-sam protein in stomach cancer, polyclonal antibody pK1-2 was raised against the extracellular domain of the gene product. This antibody detected K-sam proteins by Western blot and flow cytometry analyses in stomach cancer cell lines KATO-III and HSC39, in which the K-sam gene is amplified and overexpressed. By immunohistochemical analysis, 20 of 38 cases of the undifferentiated type of advanced stomach cancer were K-sam positive, whereas none of 11 cases of the differentiated or intestinal type revealed K-sam staining. The K-sam product was observed predominantly in diffusely infiltrative lesions. In one autopsy case, the K- sam protein was detected only locally in the primary tumor, whereas markedly increased staining for the K-sam product was detected diffusely in the metastasized tumor in the lymph node and liver. These results suggest that K- sam overexpression is associated with the malignant phenotype of the undifferentiated type of stomach cancer, such as infiltrative growth and metastasis.

Original languageEnglish
Pages (from-to)1373-1381
Number of pages9
JournalClinical Cancer Research
Volume2
Issue number8
Publication statusPublished - 1996 Aug
Externally publishedYes

Fingerprint

Stomach Neoplasms
Genes
Short Stature, Auditory Canal Atresia, Mandibular Hypoplasia, Skeletal Abnormalities
protein K
Staining and Labeling
Fibroblast Growth Factor Receptors
Cell Line
Fibroblast Growth Factors
Antibodies
Autopsy
Neoplasms
Flow Cytometry
Lymph Nodes
Western Blotting
Neoplasm Metastasis
Phenotype
Liver
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hattori, Y., Itoh, H., Uchino, S., Hosokawa, K., Ochiai, A., Ino, Y., ... Terada, M. (1996). Immunohistochemical detection of K-sam protein in stomach cancer. Clinical Cancer Research, 2(8), 1373-1381.

Immunohistochemical detection of K-sam protein in stomach cancer. / Hattori, Yutaka; Itoh, Hiroshi; Uchino, Shinya; Hosokawa, Kouichi; Ochiai, Atsushi; Ino, Yoshinori; Ishii, Hideshi; Sakamoto, Hiromi; Yamaguchi, Naohito; Yanagihara, Kazuyoshi; Hirohashi, Setsuo; Sugimura, Takashi; Terada, Masaaki.

In: Clinical Cancer Research, Vol. 2, No. 8, 08.1996, p. 1373-1381.

Research output: Contribution to journalArticle

Hattori, Y, Itoh, H, Uchino, S, Hosokawa, K, Ochiai, A, Ino, Y, Ishii, H, Sakamoto, H, Yamaguchi, N, Yanagihara, K, Hirohashi, S, Sugimura, T & Terada, M 1996, 'Immunohistochemical detection of K-sam protein in stomach cancer', Clinical Cancer Research, vol. 2, no. 8, pp. 1373-1381.
Hattori Y, Itoh H, Uchino S, Hosokawa K, Ochiai A, Ino Y et al. Immunohistochemical detection of K-sam protein in stomach cancer. Clinical Cancer Research. 1996 Aug;2(8):1373-1381.
Hattori, Yutaka ; Itoh, Hiroshi ; Uchino, Shinya ; Hosokawa, Kouichi ; Ochiai, Atsushi ; Ino, Yoshinori ; Ishii, Hideshi ; Sakamoto, Hiromi ; Yamaguchi, Naohito ; Yanagihara, Kazuyoshi ; Hirohashi, Setsuo ; Sugimura, Takashi ; Terada, Masaaki. / Immunohistochemical detection of K-sam protein in stomach cancer. In: Clinical Cancer Research. 1996 ; Vol. 2, No. 8. pp. 1373-1381.
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abstract = "The K-sam gene, originally isolated as an amplified gene from the stomach cancer cell line KATO-III, is characterized by its preferential amplification in the undifferentiated type (diffuse type) of stomach cancer and encodes one of the receptors for heparin-binding growth factors or fibroblast growth factors. The K-sam gene has been isolated by different methods and has been designated BEK, TK14, and Cek2. The receptor for keratinocyte growth factor was also found to be encoded by the same gene. To examine the expression of the K-sam protein in stomach cancer, polyclonal antibody pK1-2 was raised against the extracellular domain of the gene product. This antibody detected K-sam proteins by Western blot and flow cytometry analyses in stomach cancer cell lines KATO-III and HSC39, in which the K-sam gene is amplified and overexpressed. By immunohistochemical analysis, 20 of 38 cases of the undifferentiated type of advanced stomach cancer were K-sam positive, whereas none of 11 cases of the differentiated or intestinal type revealed K-sam staining. The K-sam product was observed predominantly in diffusely infiltrative lesions. In one autopsy case, the K- sam protein was detected only locally in the primary tumor, whereas markedly increased staining for the K-sam product was detected diffusely in the metastasized tumor in the lymph node and liver. These results suggest that K- sam overexpression is associated with the malignant phenotype of the undifferentiated type of stomach cancer, such as infiltrative growth and metastasis.",
author = "Yutaka Hattori and Hiroshi Itoh and Shinya Uchino and Kouichi Hosokawa and Atsushi Ochiai and Yoshinori Ino and Hideshi Ishii and Hiromi Sakamoto and Naohito Yamaguchi and Kazuyoshi Yanagihara and Setsuo Hirohashi and Takashi Sugimura and Masaaki Terada",
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AU - Ino, Yoshinori

AU - Ishii, Hideshi

AU - Sakamoto, Hiromi

AU - Yamaguchi, Naohito

AU - Yanagihara, Kazuyoshi

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AU - Sugimura, Takashi

AU - Terada, Masaaki

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