Immunohistochemical studies on mouse cerebral cortex grafted into the third ventricle of rats treated with cyclosporin A

Hiroshi Inoue; Shinichi Kohsaka; Kazunari Yoshida; Mitsuhiro Otani; Shigeo Toya; Yasuzo Tsukada

doi:10.1016/0304-3940(85)90507-5

Immunohistochemical studies on mouse cerebral cortex grafted into the third ventricle of rats treated with cyclosporin A

Hiroshi Inoue, Shinichi Kohsaka, Kazunari Yoshida, Mitsuhiro Otani, Shigeo Toya, Yasuzo Tsukada

Department of Neurosurgery

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Newborn mouse cerebral cortex was transplanted into the third ventricle of an adult rat brain. After the operation, the immunosuppressant cyclosporin A was administered daily for two weeks, and the grafted tissue was examined immunohistochemically at the 4th week. Neurons, astroglias and oligoden-droglias were positively stained by antibodies against neurofilament, glial fibrillary acid protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), respectively. The CNPase staining indicated that myelination had occurred in the grafted tissue. Thus both neuronal and glial elements in the grafted tissue could survive and mature in the third ventricle of the different species upon administration of cyclosporin A.

Original language	English
Pages (from-to)	289-294
Number of pages	6
Journal	Neuroscience Letters
Volume	57
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(85)90507-5
Publication status	Published - 1985 Jun 24

Keywords

2',3'-cyclic nucleotide 3'-phosphodiesterase
CNPase
GFAP
cyclosporin A
glial fibrillary acidic protein
immunohistochemistry
myelination
neural transplantation
neurofilament
rat

ASJC Scopus subject areas

Neuroscience(all)

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title = "Immunohistochemical studies on mouse cerebral cortex grafted into the third ventricle of rats treated with cyclosporin A",

abstract = "Newborn mouse cerebral cortex was transplanted into the third ventricle of an adult rat brain. After the operation, the immunosuppressant cyclosporin A was administered daily for two weeks, and the grafted tissue was examined immunohistochemically at the 4th week. Neurons, astroglias and oligoden-droglias were positively stained by antibodies against neurofilament, glial fibrillary acid protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), respectively. The CNPase staining indicated that myelination had occurred in the grafted tissue. Thus both neuronal and glial elements in the grafted tissue could survive and mature in the third ventricle of the different species upon administration of cyclosporin A.",

keywords = "2',3'-cyclic nucleotide 3'-phosphodiesterase, CNPase, GFAP, cyclosporin A, glial fibrillary acidic protein, immunohistochemistry, myelination, neural transplantation, neurofilament, rat",

author = "Hiroshi Inoue and Shinichi Kohsaka and Kazunari Yoshida and Mitsuhiro Otani and Shigeo Toya and Yasuzo Tsukada",

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AU - Inoue, Hiroshi

AU - Kohsaka, Shinichi

AU - Yoshida, Kazunari

AU - Otani, Mitsuhiro

AU - Toya, Shigeo

AU - Tsukada, Yasuzo

PY - 1985/6/24

Y1 - 1985/6/24

N2 - Newborn mouse cerebral cortex was transplanted into the third ventricle of an adult rat brain. After the operation, the immunosuppressant cyclosporin A was administered daily for two weeks, and the grafted tissue was examined immunohistochemically at the 4th week. Neurons, astroglias and oligoden-droglias were positively stained by antibodies against neurofilament, glial fibrillary acid protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), respectively. The CNPase staining indicated that myelination had occurred in the grafted tissue. Thus both neuronal and glial elements in the grafted tissue could survive and mature in the third ventricle of the different species upon administration of cyclosporin A.

AB - Newborn mouse cerebral cortex was transplanted into the third ventricle of an adult rat brain. After the operation, the immunosuppressant cyclosporin A was administered daily for two weeks, and the grafted tissue was examined immunohistochemically at the 4th week. Neurons, astroglias and oligoden-droglias were positively stained by antibodies against neurofilament, glial fibrillary acid protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), respectively. The CNPase staining indicated that myelination had occurred in the grafted tissue. Thus both neuronal and glial elements in the grafted tissue could survive and mature in the third ventricle of the different species upon administration of cyclosporin A.

KW - 2',3'-cyclic nucleotide 3'-phosphodiesterase

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KW - GFAP

KW - cyclosporin A

KW - glial fibrillary acidic protein

KW - immunohistochemistry

KW - myelination

KW - neural transplantation

KW - neurofilament

KW - rat

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