Immunohistochemistry for aldosterone synthase CYP11B2 and matrix-assisted laser desorption ionization imaging mass spectrometry for in-situ aldosterone detection

Zhen Zhang, Yuki Sugiura, Tomoatsu Mune, Mitsuru Nishiyama, Yoshio Terada, Kuniaki Mukai, Koshiro Nishimoto

Research output: Contribution to journalReview articlepeer-review

4 Citations (Scopus)

Abstract

Purpose of reviewImmunohistochemistry for aldosterone synthase (CYP11B2) has markedly provided a comprehensive picture of the adrenocortical diseases, particularly primary aldosteronism. The findings from CYP11B2-immunohistochemistry are consistent with the clinical courses of most patients with primary aldosteronism. We herein review the updated pathophysiology and usefulness of the method for understanding individual patients with different subtypes of primary aldosteronism.Recent findingsAfter our discovery of aldosterone-producing cell clusters (APCCs) using the immunohistochemistry for CYP11B2, we found possible APCC-to-APA transitional lesions (pAATLs) in a few cases that had been hitherto classified as unilateral hyperplasia or multiple nodules. On the basis of morphological and functional features of pAATLs as well as distributions of somatic mutations within the lesions, we have made a hypothesis that APCC grows to APA via pAATL for one of developmental courses of APA. Recently, we successfully performed in-situ detection of aldosterone on adrenal tissue sections using a state-of-the-art technique, matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-imaging). This method revealed in-situ synthesis of aldosterone in APCCs and APAs in addition to several other steroids.SummaryCYP11B2 immunohistochemistry revealed the pathophysiology of aldosterone production in the past decade, especially formation of APCC in normal adrenals and pAATL that is a possible lesion developing from APCC to APA. The term 'idiopathic hyperaldosteronism' may soon become obsolete.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalCurrent opinion in nephrology and hypertension
Volume28
Issue number2
DOIs
Publication statusPublished - 2019 Mar 1

Keywords

  • aldosterone
  • aldosterone synthase (CYP11B2)
  • aldosterone-producing adenomas
  • aldosterone-producing cell clusters
  • matrix-assisted laser desorption ionization imaging mass spectrometry

ASJC Scopus subject areas

  • Internal Medicine
  • Nephrology

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