Immunolocalization of hepatocyte growth factor and its receptor (c-Met) during mouse liver development

Kiyoshi S. Ishikawa, Toru Masui, Katsutoshi Ishikawa, Nobuyoshi Shiojiri

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Although hepatocyte growth factor (HGF) was discovered as a potent hepatotrophic factor responsible for liver regeneration and may involve some organ development in embryogenesis, it remains to be revealed what roles HGF plays in liver development. The present study was undertaken to determine which cells express HGF and its receptor c-Met and when c-Met is activated in mouse liver development by using immunoblotting and immunohistochemical techniques. HGF was detected in hepatocytes and non-parenchymal cells, including biliary epithelial cells, periportal connective tissue cells, megakaryocytes, endothelial cells, and sinusoidal cells, throughout liver development. Positive HGF immunostaining in hepatocytes increased during postnatal development, and reached the maximal level in the adult stage. c-Met protein was also expressed in hepatocytes throughout liver development, but maximal staining was obtained in 1- or 2-week-old livers. Phosphorylation of tyrosine residues in the c-Met β chain also occurred in these stages. These results suggest that HGF signaling is implicated in hepatocyte growth during postnatal liver development, and its action could be in a paracrine mode; HGF produced by non-parenchymal cells such as sinusoidal cells acts on hepatocytes expressing c-Met receptors. Positive immunostaining in adult and postnatal hepatocytes may be derived from their blood clearance of HGF.

Original languageEnglish
Pages (from-to)453-462
Number of pages10
JournalHistochemistry and Cell Biology
Volume116
Issue number5
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins c-met
Hepatocyte Growth Factor
liver
Liver
mice
Hepatocytes
cells
Connective Tissue Cells
Liver Regeneration
Megakaryocytes
connective tissue
Intercellular Signaling Peptides and Proteins
Immunoblotting
phosphorylation
Embryonic Development
tyrosine
Tyrosine
clearances
staining
Endothelial Cells

Keywords

  • c-Met
  • Growth
  • Hepatocytes
  • HGF
  • Mouse

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Immunolocalization of hepatocyte growth factor and its receptor (c-Met) during mouse liver development. / Ishikawa, Kiyoshi S.; Masui, Toru; Ishikawa, Katsutoshi; Shiojiri, Nobuyoshi.

In: Histochemistry and Cell Biology, Vol. 116, No. 5, 2001, p. 453-462.

Research output: Contribution to journalArticle

Ishikawa, Kiyoshi S. ; Masui, Toru ; Ishikawa, Katsutoshi ; Shiojiri, Nobuyoshi. / Immunolocalization of hepatocyte growth factor and its receptor (c-Met) during mouse liver development. In: Histochemistry and Cell Biology. 2001 ; Vol. 116, No. 5. pp. 453-462.
@article{e53bc60db80541e5a3db2b05f3fb1eed,
title = "Immunolocalization of hepatocyte growth factor and its receptor (c-Met) during mouse liver development",
abstract = "Although hepatocyte growth factor (HGF) was discovered as a potent hepatotrophic factor responsible for liver regeneration and may involve some organ development in embryogenesis, it remains to be revealed what roles HGF plays in liver development. The present study was undertaken to determine which cells express HGF and its receptor c-Met and when c-Met is activated in mouse liver development by using immunoblotting and immunohistochemical techniques. HGF was detected in hepatocytes and non-parenchymal cells, including biliary epithelial cells, periportal connective tissue cells, megakaryocytes, endothelial cells, and sinusoidal cells, throughout liver development. Positive HGF immunostaining in hepatocytes increased during postnatal development, and reached the maximal level in the adult stage. c-Met protein was also expressed in hepatocytes throughout liver development, but maximal staining was obtained in 1- or 2-week-old livers. Phosphorylation of tyrosine residues in the c-Met β chain also occurred in these stages. These results suggest that HGF signaling is implicated in hepatocyte growth during postnatal liver development, and its action could be in a paracrine mode; HGF produced by non-parenchymal cells such as sinusoidal cells acts on hepatocytes expressing c-Met receptors. Positive immunostaining in adult and postnatal hepatocytes may be derived from their blood clearance of HGF.",
keywords = "c-Met, Growth, Hepatocytes, HGF, Mouse",
author = "Ishikawa, {Kiyoshi S.} and Toru Masui and Katsutoshi Ishikawa and Nobuyoshi Shiojiri",
year = "2001",
doi = "10.1007/s00418-001-0342-6",
language = "English",
volume = "116",
pages = "453--462",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Immunolocalization of hepatocyte growth factor and its receptor (c-Met) during mouse liver development

AU - Ishikawa, Kiyoshi S.

AU - Masui, Toru

AU - Ishikawa, Katsutoshi

AU - Shiojiri, Nobuyoshi

PY - 2001

Y1 - 2001

N2 - Although hepatocyte growth factor (HGF) was discovered as a potent hepatotrophic factor responsible for liver regeneration and may involve some organ development in embryogenesis, it remains to be revealed what roles HGF plays in liver development. The present study was undertaken to determine which cells express HGF and its receptor c-Met and when c-Met is activated in mouse liver development by using immunoblotting and immunohistochemical techniques. HGF was detected in hepatocytes and non-parenchymal cells, including biliary epithelial cells, periportal connective tissue cells, megakaryocytes, endothelial cells, and sinusoidal cells, throughout liver development. Positive HGF immunostaining in hepatocytes increased during postnatal development, and reached the maximal level in the adult stage. c-Met protein was also expressed in hepatocytes throughout liver development, but maximal staining was obtained in 1- or 2-week-old livers. Phosphorylation of tyrosine residues in the c-Met β chain also occurred in these stages. These results suggest that HGF signaling is implicated in hepatocyte growth during postnatal liver development, and its action could be in a paracrine mode; HGF produced by non-parenchymal cells such as sinusoidal cells acts on hepatocytes expressing c-Met receptors. Positive immunostaining in adult and postnatal hepatocytes may be derived from their blood clearance of HGF.

AB - Although hepatocyte growth factor (HGF) was discovered as a potent hepatotrophic factor responsible for liver regeneration and may involve some organ development in embryogenesis, it remains to be revealed what roles HGF plays in liver development. The present study was undertaken to determine which cells express HGF and its receptor c-Met and when c-Met is activated in mouse liver development by using immunoblotting and immunohistochemical techniques. HGF was detected in hepatocytes and non-parenchymal cells, including biliary epithelial cells, periportal connective tissue cells, megakaryocytes, endothelial cells, and sinusoidal cells, throughout liver development. Positive HGF immunostaining in hepatocytes increased during postnatal development, and reached the maximal level in the adult stage. c-Met protein was also expressed in hepatocytes throughout liver development, but maximal staining was obtained in 1- or 2-week-old livers. Phosphorylation of tyrosine residues in the c-Met β chain also occurred in these stages. These results suggest that HGF signaling is implicated in hepatocyte growth during postnatal liver development, and its action could be in a paracrine mode; HGF produced by non-parenchymal cells such as sinusoidal cells acts on hepatocytes expressing c-Met receptors. Positive immunostaining in adult and postnatal hepatocytes may be derived from their blood clearance of HGF.

KW - c-Met

KW - Growth

KW - Hepatocytes

KW - HGF

KW - Mouse

UR - http://www.scopus.com/inward/record.url?scp=0035208270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035208270&partnerID=8YFLogxK

U2 - 10.1007/s00418-001-0342-6

DO - 10.1007/s00418-001-0342-6

M3 - Article

VL - 116

SP - 453

EP - 462

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 5

ER -