Immunolocalization of matrix metalloproteinase 3 (stromelysin) in rheumatoid synovioblasts (B cells): Correlation with rheumatoid arthritis

Yasunori Okada, Naoto Takeuchi, Katsuro Tomita, Isao Nakanishi, Hideaki Nagase

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Metalloproteinases produced by connective tissue cells may play a key part in the destruction of joints in rheumatoid arthritis. Matrix metalloproteinase 3 (MMP-3; stromelysin) capable of degrading cartilage proteoglycans and type IX collagen and of activating procollagenase was immunolocalised in hyperplastic synovial lining cells in rheumatoid synovium, but not in the cells of normal synovium. Cells responsible for synthesis of MMP-3 have the phenotype of synovioblasts (B cells) by immunoelectron microscopy, but not of phagocytic synovial macrophages (A cells). Cultured monolayer of rheumatoid synovial cells synthesises MMP-3 only under treatment with macrophage conditioned medium. Immunolocalisation of MMP-3 in rheumatoid synovium and cultured synovial cells was possible when the specimens were treated with a monovalent ionophore, monensin. These results suggest that MMP-3 is synthesised and secreted continuously without storage from hyperplastic synovioblasts stimulated by factor(s) derived from activated macrophages present in the synovium.

Original languageEnglish
Pages (from-to)645-653
Number of pages9
JournalAnnals of the Rheumatic Diseases
Volume48
Issue number8
DOIs
Publication statusPublished - 1989

Fingerprint

Matrix Metalloproteinase 3
Matrix Metalloproteinases
Synovial Membrane
Rheumatoid Arthritis
B-Lymphocytes
Cells
Macrophages
Matrix Metalloproteinase 11
Collagen Type IX
Connective Tissue Cells
Monensin
Immunoelectron Microscopy
Ionophores
Cartilage
Metalloproteases
Proteoglycans
Conditioned Culture Medium
Linings
Cultured Cells
Monolayers

ASJC Scopus subject areas

  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Ophthalmology
  • Immunology
  • Rheumatology

Cite this

Immunolocalization of matrix metalloproteinase 3 (stromelysin) in rheumatoid synovioblasts (B cells) : Correlation with rheumatoid arthritis. / Okada, Yasunori; Takeuchi, Naoto; Tomita, Katsuro; Nakanishi, Isao; Nagase, Hideaki.

In: Annals of the Rheumatic Diseases, Vol. 48, No. 8, 1989, p. 645-653.

Research output: Contribution to journalArticle

Okada, Yasunori ; Takeuchi, Naoto ; Tomita, Katsuro ; Nakanishi, Isao ; Nagase, Hideaki. / Immunolocalization of matrix metalloproteinase 3 (stromelysin) in rheumatoid synovioblasts (B cells) : Correlation with rheumatoid arthritis. In: Annals of the Rheumatic Diseases. 1989 ; Vol. 48, No. 8. pp. 645-653.
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