Immunolocalization of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomas

A. Kawashima, Y. Okada, I. Nakanishi, Y. Ueda, K. Iwata, A. Roessner

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have immunohistochemically examined the localization of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in human chondrosarcomas (CHS) (23 cases) and benign chondroid lesions (BCL) (16 cases of osteochondromas and 11 cases of enchondromas). In CHS, all the MMPs and TIMPs examined were positive. Among them, MMP-1 was immunolocalized in more than 90% of both CHS and BCL, but positive score of MMP-1 was significantly higher in CHS than that in BCL (p < 0.01). Compared with BCL, CHS expressed MMP-3 at a low level, and more often positive in MMP-9. It is possible that chondrosarcoma might have a tendency to lose the ability to secrete MMP-3, which is a metalloproteinase that can degrade cartilage proteoglycans and is related to normal cartilage turnover. MMP-2, TIMP-1 and TIMP-2 were immunolocalized in more than 70% of the cases of both BCL and CHS, but the positive scores of these were not statistically different between the two groups. Interestingly, in several cases of CHS, both MMP-1 and MMP-9 immunostains were observed preferentially within the cells at the marginal areas of cartilaginous lobules. These findings suggest that increased expression of MMP-1 and MMP-9 and decrease in MMP-3 expression are associated with the malignant phenotype of the cartilaginour tumors.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalGeneral and Diagnostic Pathology
Volume142
Issue number3-4
Publication statusPublished - 1997

Fingerprint

Tissue Inhibitor of Metalloproteinases
Chondrosarcoma
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 1
Matrix Metalloproteinase 3
Matrix Metalloproteinase 9
Matrix Metalloproteinase 2
Matrix Metalloproteinases
Cartilage
Osteochondroma
Chondroma
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinase-1
Metalloproteases
Proteoglycans
Phenotype

Keywords

  • Chondrosarcoma
  • Enchondroma
  • Immunohistochemistry
  • Matrix metalloproteinase
  • Osteochondroma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Kawashima, A., Okada, Y., Nakanishi, I., Ueda, Y., Iwata, K., & Roessner, A. (1997). Immunolocalization of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomas. General and Diagnostic Pathology, 142(3-4), 129-137.

Immunolocalization of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomas. / Kawashima, A.; Okada, Y.; Nakanishi, I.; Ueda, Y.; Iwata, K.; Roessner, A.

In: General and Diagnostic Pathology, Vol. 142, No. 3-4, 1997, p. 129-137.

Research output: Contribution to journalArticle

Kawashima, A, Okada, Y, Nakanishi, I, Ueda, Y, Iwata, K & Roessner, A 1997, 'Immunolocalization of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomas', General and Diagnostic Pathology, vol. 142, no. 3-4, pp. 129-137.
Kawashima, A. ; Okada, Y. ; Nakanishi, I. ; Ueda, Y. ; Iwata, K. ; Roessner, A. / Immunolocalization of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomas. In: General and Diagnostic Pathology. 1997 ; Vol. 142, No. 3-4. pp. 129-137.
@article{60b331a4362f41878dfac275e6ed77c4,
title = "Immunolocalization of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomas",
abstract = "We have immunohistochemically examined the localization of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in human chondrosarcomas (CHS) (23 cases) and benign chondroid lesions (BCL) (16 cases of osteochondromas and 11 cases of enchondromas). In CHS, all the MMPs and TIMPs examined were positive. Among them, MMP-1 was immunolocalized in more than 90{\%} of both CHS and BCL, but positive score of MMP-1 was significantly higher in CHS than that in BCL (p < 0.01). Compared with BCL, CHS expressed MMP-3 at a low level, and more often positive in MMP-9. It is possible that chondrosarcoma might have a tendency to lose the ability to secrete MMP-3, which is a metalloproteinase that can degrade cartilage proteoglycans and is related to normal cartilage turnover. MMP-2, TIMP-1 and TIMP-2 were immunolocalized in more than 70{\%} of the cases of both BCL and CHS, but the positive scores of these were not statistically different between the two groups. Interestingly, in several cases of CHS, both MMP-1 and MMP-9 immunostains were observed preferentially within the cells at the marginal areas of cartilaginous lobules. These findings suggest that increased expression of MMP-1 and MMP-9 and decrease in MMP-3 expression are associated with the malignant phenotype of the cartilaginour tumors.",
keywords = "Chondrosarcoma, Enchondroma, Immunohistochemistry, Matrix metalloproteinase, Osteochondroma",
author = "A. Kawashima and Y. Okada and I. Nakanishi and Y. Ueda and K. Iwata and A. Roessner",
year = "1997",
language = "English",
volume = "142",
pages = "129--137",
journal = "General and Diagnostic Pathology",
issn = "0947-823X",
publisher = "Gustav Fischer Verlag",
number = "3-4",

}

TY - JOUR

T1 - Immunolocalization of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomas

AU - Kawashima, A.

AU - Okada, Y.

AU - Nakanishi, I.

AU - Ueda, Y.

AU - Iwata, K.

AU - Roessner, A.

PY - 1997

Y1 - 1997

N2 - We have immunohistochemically examined the localization of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in human chondrosarcomas (CHS) (23 cases) and benign chondroid lesions (BCL) (16 cases of osteochondromas and 11 cases of enchondromas). In CHS, all the MMPs and TIMPs examined were positive. Among them, MMP-1 was immunolocalized in more than 90% of both CHS and BCL, but positive score of MMP-1 was significantly higher in CHS than that in BCL (p < 0.01). Compared with BCL, CHS expressed MMP-3 at a low level, and more often positive in MMP-9. It is possible that chondrosarcoma might have a tendency to lose the ability to secrete MMP-3, which is a metalloproteinase that can degrade cartilage proteoglycans and is related to normal cartilage turnover. MMP-2, TIMP-1 and TIMP-2 were immunolocalized in more than 70% of the cases of both BCL and CHS, but the positive scores of these were not statistically different between the two groups. Interestingly, in several cases of CHS, both MMP-1 and MMP-9 immunostains were observed preferentially within the cells at the marginal areas of cartilaginous lobules. These findings suggest that increased expression of MMP-1 and MMP-9 and decrease in MMP-3 expression are associated with the malignant phenotype of the cartilaginour tumors.

AB - We have immunohistochemically examined the localization of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in human chondrosarcomas (CHS) (23 cases) and benign chondroid lesions (BCL) (16 cases of osteochondromas and 11 cases of enchondromas). In CHS, all the MMPs and TIMPs examined were positive. Among them, MMP-1 was immunolocalized in more than 90% of both CHS and BCL, but positive score of MMP-1 was significantly higher in CHS than that in BCL (p < 0.01). Compared with BCL, CHS expressed MMP-3 at a low level, and more often positive in MMP-9. It is possible that chondrosarcoma might have a tendency to lose the ability to secrete MMP-3, which is a metalloproteinase that can degrade cartilage proteoglycans and is related to normal cartilage turnover. MMP-2, TIMP-1 and TIMP-2 were immunolocalized in more than 70% of the cases of both BCL and CHS, but the positive scores of these were not statistically different between the two groups. Interestingly, in several cases of CHS, both MMP-1 and MMP-9 immunostains were observed preferentially within the cells at the marginal areas of cartilaginous lobules. These findings suggest that increased expression of MMP-1 and MMP-9 and decrease in MMP-3 expression are associated with the malignant phenotype of the cartilaginour tumors.

KW - Chondrosarcoma

KW - Enchondroma

KW - Immunohistochemistry

KW - Matrix metalloproteinase

KW - Osteochondroma

UR - http://www.scopus.com/inward/record.url?scp=0031056645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031056645&partnerID=8YFLogxK

M3 - Article

C2 - 9065576

AN - SCOPUS:0031056645

VL - 142

SP - 129

EP - 137

JO - General and Diagnostic Pathology

JF - General and Diagnostic Pathology

SN - 0947-823X

IS - 3-4

ER -