Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma

Yoshito Numata; Keiji Dohi; Ayako Furukawa; Shino Kikuoka; Hidehisa Asada; Takahiro Fukunaga; Yasushi Taniguchi; Kazuyuki Sasakura; Tetsuo Tsuji; Ken Inouye; Michihiro Yoshimura; Hiroshi Itoh; Masashi Mukoyama; Hirofumi Yasue; Kazuwa Nakao

doi:10.1093/clinchem/44.5.1008

Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma

Yoshito Numata, Keiji Dohi, Ayako Furukawa, Shino Kikuoka, Hidehisa Asada, Takahiro Fukunaga, Yasushi Taniguchi, Kazuyuki Sasakura, Tetsuo Tsuji, Ken Inouye, Michihiro Yoshimura, Hiroshi Itoh, Masashi Mukoyama, Hirofumi Yasue, Kazuwa Nakao

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Recently, the N-terminal fragment of proatrial natriuretic peptide (N- terminal proANP) has been proposed as a marker of chronic congestive heart failure. In this study, we established a two-step immunoradiometric assay using monoclonal antibodies and synthetic N-terminal proANP (1-67) as a standard. It allows us to measure plasma N-terminal proANP in only 4 h without prior extraction. The detection limit of this assay was 15 pmol/L for a 100 μL sample of plasma. Within-run CVs ranged from 1.7% to 2.9% and between-run CVs ranged from 4.2% to 5.1%. The dilution curves of plasma samples showed good linearity and analytical recovery was 89-104%. The mean (±SD) N-terminal proANP in plasma of 33 healthy subjects was 188 (±71) pmol/L and 1030 (±411) pmol/L in 25 patients with heart failure. Our immunoradiometric assay is rapid and precise enough for routine determination of N-terminal proANP in human plasma.

Original language	English
Pages (from-to)	1008-1013
Number of pages	6
Journal	Clinical Chemistry
Volume	44
Issue number	5
DOIs	https://doi.org/10.1093/clinchem/44.5.1008
Publication status	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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Numata, Y., Dohi, K., Furukawa, A., Kikuoka, S., Asada, H., Fukunaga, T., Taniguchi, Y., Sasakura, K., Tsuji, T., Inouye, K., Yoshimura, M., Itoh, H., Mukoyama, M., Yasue, H., & Nakao, K. (1998). Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma. Clinical Chemistry, 44(5), 1008-1013. https://doi.org/10.1093/clinchem/44.5.1008

Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma. / Numata, Yoshito; Dohi, Keiji; Furukawa, Ayako; Kikuoka, Shino; Asada, Hidehisa; Fukunaga, Takahiro; Taniguchi, Yasushi; Sasakura, Kazuyuki; Tsuji, Tetsuo; Inouye, Ken; Yoshimura, Michihiro; Itoh, Hiroshi; Mukoyama, Masashi; Yasue, Hirofumi; Nakao, Kazuwa.

In: Clinical Chemistry, Vol. 44, No. 5, 1998, p. 1008-1013.

Research output: Contribution to journal › Article › peer-review

Numata, Y, Dohi, K, Furukawa, A, Kikuoka, S, Asada, H, Fukunaga, T, Taniguchi, Y, Sasakura, K, Tsuji, T, Inouye, K, Yoshimura, M, Itoh, H, Mukoyama, M, Yasue, H & Nakao, K 1998, 'Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma', Clinical Chemistry, vol. 44, no. 5, pp. 1008-1013. https://doi.org/10.1093/clinchem/44.5.1008

Numata, Yoshito ; Dohi, Keiji ; Furukawa, Ayako ; Kikuoka, Shino ; Asada, Hidehisa ; Fukunaga, Takahiro ; Taniguchi, Yasushi ; Sasakura, Kazuyuki ; Tsuji, Tetsuo ; Inouye, Ken ; Yoshimura, Michihiro ; Itoh, Hiroshi ; Mukoyama, Masashi ; Yasue, Hirofumi ; Nakao, Kazuwa. / Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma. In: Clinical Chemistry. 1998 ; Vol. 44, No. 5. pp. 1008-1013.

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abstract = "Recently, the N-terminal fragment of proatrial natriuretic peptide (N- terminal proANP) has been proposed as a marker of chronic congestive heart failure. In this study, we established a two-step immunoradiometric assay using monoclonal antibodies and synthetic N-terminal proANP (1-67) as a standard. It allows us to measure plasma N-terminal proANP in only 4 h without prior extraction. The detection limit of this assay was 15 pmol/L for a 100 μL sample of plasma. Within-run CVs ranged from 1.7% to 2.9% and between-run CVs ranged from 4.2% to 5.1%. The dilution curves of plasma samples showed good linearity and analytical recovery was 89-104%. The mean (±SD) N-terminal proANP in plasma of 33 healthy subjects was 188 (±71) pmol/L and 1030 (±411) pmol/L in 25 patients with heart failure. Our immunoradiometric assay is rapid and precise enough for routine determination of N-terminal proANP in human plasma.",

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AU - Asada, Hidehisa

AU - Fukunaga, Takahiro

AU - Taniguchi, Yasushi

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AU - Tsuji, Tetsuo

AU - Inouye, Ken

AU - Yoshimura, Michihiro

AU - Itoh, Hiroshi

AU - Mukoyama, Masashi

AU - Yasue, Hirofumi

AU - Nakao, Kazuwa

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