Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma

Yoshito Numata, Keiji Dohi, Ayako Furukawa, Shino Kikuoka, Hidehisa Asada, Takahiro Fukunaga, Yasushi Taniguchi, Kazuyuki Sasakura, Tetsuo Tsuji, Ken Inouye, Michihiro Yoshimura, Hiroshi Itoh, Masashi Mukoyama, Hirofumi Yasue, Kazuwa Nakao

Research output: Contribution to journalArticle

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Abstract

Recently, the N-terminal fragment of proatrial natriuretic peptide (N- terminal proANP) has been proposed as a marker of chronic congestive heart failure. In this study, we established a two-step immunoradiometric assay using monoclonal antibodies and synthetic N-terminal proANP (1-67) as a standard. It allows us to measure plasma N-terminal proANP in only 4 h without prior extraction. The detection limit of this assay was 15 pmol/L for a 100 μL sample of plasma. Within-run CVs ranged from 1.7% to 2.9% and between-run CVs ranged from 4.2% to 5.1%. The dilution curves of plasma samples showed good linearity and analytical recovery was 89-104%. The mean (±SD) N-terminal proANP in plasma of 33 healthy subjects was 188 (±71) pmol/L and 1030 (±411) pmol/L in 25 patients with heart failure. Our immunoradiometric assay is rapid and precise enough for routine determination of N-terminal proANP in human plasma.

Original languageEnglish
Pages (from-to)1008-1013
Number of pages6
JournalClinical Chemistry
Volume44
Issue number5
Publication statusPublished - 1998
Externally publishedYes

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Plasma (human)
Immunoradiometric Assay
Natriuretic Peptides
Assays
Plasmas
Heart Failure
Dilution
Monoclonal Antibodies
Limit of Detection
Healthy Volunteers
Recovery
N-terminal proatrial natriuretic peptide

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Numata, Y., Dohi, K., Furukawa, A., Kikuoka, S., Asada, H., Fukunaga, T., ... Nakao, K. (1998). Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma. Clinical Chemistry, 44(5), 1008-1013.

Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma. / Numata, Yoshito; Dohi, Keiji; Furukawa, Ayako; Kikuoka, Shino; Asada, Hidehisa; Fukunaga, Takahiro; Taniguchi, Yasushi; Sasakura, Kazuyuki; Tsuji, Tetsuo; Inouye, Ken; Yoshimura, Michihiro; Itoh, Hiroshi; Mukoyama, Masashi; Yasue, Hirofumi; Nakao, Kazuwa.

In: Clinical Chemistry, Vol. 44, No. 5, 1998, p. 1008-1013.

Research output: Contribution to journalArticle

Numata, Y, Dohi, K, Furukawa, A, Kikuoka, S, Asada, H, Fukunaga, T, Taniguchi, Y, Sasakura, K, Tsuji, T, Inouye, K, Yoshimura, M, Itoh, H, Mukoyama, M, Yasue, H & Nakao, K 1998, 'Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma', Clinical Chemistry, vol. 44, no. 5, pp. 1008-1013.
Numata Y, Dohi K, Furukawa A, Kikuoka S, Asada H, Fukunaga T et al. Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma. Clinical Chemistry. 1998;44(5):1008-1013.
Numata, Yoshito ; Dohi, Keiji ; Furukawa, Ayako ; Kikuoka, Shino ; Asada, Hidehisa ; Fukunaga, Takahiro ; Taniguchi, Yasushi ; Sasakura, Kazuyuki ; Tsuji, Tetsuo ; Inouye, Ken ; Yoshimura, Michihiro ; Itoh, Hiroshi ; Mukoyama, Masashi ; Yasue, Hirofumi ; Nakao, Kazuwa. / Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma. In: Clinical Chemistry. 1998 ; Vol. 44, No. 5. pp. 1008-1013.
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AU - Asada, Hidehisa

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AU - Yasue, Hirofumi

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