Abstract
Recently, the N-terminal fragment of proatrial natriuretic peptide (N- terminal proANP) has been proposed as a marker of chronic congestive heart failure. In this study, we established a two-step immunoradiometric assay using monoclonal antibodies and synthetic N-terminal proANP (1-67) as a standard. It allows us to measure plasma N-terminal proANP in only 4 h without prior extraction. The detection limit of this assay was 15 pmol/L for a 100 μL sample of plasma. Within-run CVs ranged from 1.7% to 2.9% and between-run CVs ranged from 4.2% to 5.1%. The dilution curves of plasma samples showed good linearity and analytical recovery was 89-104%. The mean (±SD) N-terminal proANP in plasma of 33 healthy subjects was 188 (±71) pmol/L and 1030 (±411) pmol/L in 25 patients with heart failure. Our immunoradiometric assay is rapid and precise enough for routine determination of N-terminal proANP in human plasma.
| Original language | English |
|---|---|
| Pages (from-to) | 1008-1013 |
| Number of pages | 6 |
| Journal | Clinical Chemistry |
| Volume | 44 |
| Issue number | 5 |
| Publication status | Published - 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma. / Numata, Yoshito; Dohi, Keiji; Furukawa, Ayako; Kikuoka, Shino; Asada, Hidehisa; Fukunaga, Takahiro; Taniguchi, Yasushi; Sasakura, Kazuyuki; Tsuji, Tetsuo; Inouye, Ken; Yoshimura, Michihiro; Itoh, Hiroshi; Mukoyama, Masashi; Yasue, Hirofumi; Nakao, Kazuwa.
In: Clinical Chemistry, Vol. 44, No. 5, 1998, p. 1008-1013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma
AU - Numata, Yoshito
AU - Dohi, Keiji
AU - Furukawa, Ayako
AU - Kikuoka, Shino
AU - Asada, Hidehisa
AU - Fukunaga, Takahiro
AU - Taniguchi, Yasushi
AU - Sasakura, Kazuyuki
AU - Tsuji, Tetsuo
AU - Inouye, Ken
AU - Yoshimura, Michihiro
AU - Itoh, Hiroshi
AU - Mukoyama, Masashi
AU - Yasue, Hirofumi
AU - Nakao, Kazuwa
PY - 1998
Y1 - 1998
N2 - Recently, the N-terminal fragment of proatrial natriuretic peptide (N- terminal proANP) has been proposed as a marker of chronic congestive heart failure. In this study, we established a two-step immunoradiometric assay using monoclonal antibodies and synthetic N-terminal proANP (1-67) as a standard. It allows us to measure plasma N-terminal proANP in only 4 h without prior extraction. The detection limit of this assay was 15 pmol/L for a 100 μL sample of plasma. Within-run CVs ranged from 1.7% to 2.9% and between-run CVs ranged from 4.2% to 5.1%. The dilution curves of plasma samples showed good linearity and analytical recovery was 89-104%. The mean (±SD) N-terminal proANP in plasma of 33 healthy subjects was 188 (±71) pmol/L and 1030 (±411) pmol/L in 25 patients with heart failure. Our immunoradiometric assay is rapid and precise enough for routine determination of N-terminal proANP in human plasma.
AB - Recently, the N-terminal fragment of proatrial natriuretic peptide (N- terminal proANP) has been proposed as a marker of chronic congestive heart failure. In this study, we established a two-step immunoradiometric assay using monoclonal antibodies and synthetic N-terminal proANP (1-67) as a standard. It allows us to measure plasma N-terminal proANP in only 4 h without prior extraction. The detection limit of this assay was 15 pmol/L for a 100 μL sample of plasma. Within-run CVs ranged from 1.7% to 2.9% and between-run CVs ranged from 4.2% to 5.1%. The dilution curves of plasma samples showed good linearity and analytical recovery was 89-104%. The mean (±SD) N-terminal proANP in plasma of 33 healthy subjects was 188 (±71) pmol/L and 1030 (±411) pmol/L in 25 patients with heart failure. Our immunoradiometric assay is rapid and precise enough for routine determination of N-terminal proANP in human plasma.
UR - http://www.scopus.com/inward/record.url?scp=15444344202&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=15444344202&partnerID=8YFLogxK
M3 - Article
C2 - 9590374
AN - SCOPUS:15444344202
VL - 44
SP - 1008
EP - 1013
JO - Clinical Chemistry
JF - Clinical Chemistry
SN - 0009-9147
IS - 5
ER -