Immunoreactivity against intracellular domains of desmogleins in pemphigus

Yoshiyuki Ohata, Masayuki Amagai, Ken Ishii, Takashi Hashimoto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In pemphigus vulgaris (PV) and pemphigus foliaceus (PF), most of the autoantibodies direct against the extracellular domains of desmoglein 1 (Dsg1) or Dsg3, and those antibodies are proved to play a pathogenic role in blister formation in the skin and mucous membranes. However, some pemphigus sera have been reported to react with the intracellular domains of these antigens. In the present study, we examined the reactivity of the sera from various types of pemphigus with recombinant proteins of extracellular and intracellular domains of human Dsg1 and Dsg3 by immunoblot analysis. We produced the entire extracellular domain of Dsg1 or Dsg3 fused with mouse IgG2a by baculovirus expression. We prepared the intracellular domain of Dsg1 or Dsg3 fused with glutathione-S-transferase by bacterial expression. All of the 31 PV sera reacted with the extracellular domain of Dsg3 and four reacted with the intracellular domain. Six out of 19 PF sera reacted with the extracellular domain of Dsg1 and five reacted with the intracellular domain. In addition, some sera of Brazilian PF patients or cases with mixed features of PV and PF also reacted with the intracellular domains of Dsg1 or Dsg3. Although the frequency was low, some sera did react with the intracellular domain of Dsgs.

Original languageEnglish
Pages (from-to)64-71
Number of pages8
JournalJournal of Dermatological Science
Volume25
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Desmogleins
Desmoglein 1
Pemphigus
Serum
Glutathione Transferase
Recombinant Proteins
Autoantibodies
Baculoviridae
Skin
Blister
Antigens
Mucous Membrane
Antibodies

Keywords

  • Desmoglein
  • Epitope
  • Immunoblotting
  • Pemphigus foliaceus
  • Pemphigus vulgaris

ASJC Scopus subject areas

  • Dermatology

Cite this

Immunoreactivity against intracellular domains of desmogleins in pemphigus. / Ohata, Yoshiyuki; Amagai, Masayuki; Ishii, Ken; Hashimoto, Takashi.

In: Journal of Dermatological Science, Vol. 25, No. 1, 2001, p. 64-71.

Research output: Contribution to journalArticle

Ohata, Yoshiyuki ; Amagai, Masayuki ; Ishii, Ken ; Hashimoto, Takashi. / Immunoreactivity against intracellular domains of desmogleins in pemphigus. In: Journal of Dermatological Science. 2001 ; Vol. 25, No. 1. pp. 64-71.
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