Immunosenescent colitogenic CD4+ T cells convert to regulatory cells and suppress colitis

Teruji Totsuka, Takanori Kanai, Yasuhiro Nemoto, Takayuki Tomita, Kiichiro Tsuchiya, Naoya Sakamoto, Ryuichi Okamoto, Mamoru Watanabe

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Inflammatory bowel diseases progress steadily by the expansion of colitogenic CD4+ cells. However, it remains unknown whether colitogenic CD4+ cells are long-living like memory cells or exhausted like effector cells. To assess the longevity of colitogenic lamina propria (LP) CD4+ cells, we performed sequential transfers of LP CD4+ cells from colitic CD4+CD45RBhigh cell-transferred SCID mice into new SCID mice. Although SCID mice transferred with colitic LP CD4+ cells stably developed colitis until at least the sixth transfer, the interval to the development of colitis gradually lengthened as the number of transfers increased. The incidence of colitis gradually decreased after the seventh transfer. Furthermore, non-colitic LP CD4+ cells from mice transferred over seven times expressed significantly higher levels of PD-1 and produced significantly lower amounts of IFN-γ, TNF-α, and IL-17 than colitic LP CD4+ cells recovered after the first transfer. Most notably, we found that re-transfer of non-colitic LP CD4+ cells recovered after multiple transfers prevented the development of colitis in SCID mice co-transferred with CD4+CD45RBhigh cells. Thus, colitogenic LP CD4+ cells may be exhausted over time, become non-functional, convert to regulatory cells, and finally suppress colitis in the process of immunosenescence.

Original languageEnglish
Pages (from-to)1275-1286
Number of pages12
JournalEuropean Journal of Immunology
Volume38
Issue number5
DOIs
Publication statusPublished - 2008 May
Externally publishedYes

Fingerprint

Colitis
T-Lymphocytes
Mucous Membrane
SCID Mice
Interleukin-17
Inflammatory Bowel Diseases

Keywords

  • Animal models
  • CD4 T cells
  • Intestinal immunity
  • Mucosal immunity

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Totsuka, T., Kanai, T., Nemoto, Y., Tomita, T., Tsuchiya, K., Sakamoto, N., ... Watanabe, M. (2008). Immunosenescent colitogenic CD4+ T cells convert to regulatory cells and suppress colitis. European Journal of Immunology, 38(5), 1275-1286. https://doi.org/10.1002/eji.200737914

Immunosenescent colitogenic CD4+ T cells convert to regulatory cells and suppress colitis. / Totsuka, Teruji; Kanai, Takanori; Nemoto, Yasuhiro; Tomita, Takayuki; Tsuchiya, Kiichiro; Sakamoto, Naoya; Okamoto, Ryuichi; Watanabe, Mamoru.

In: European Journal of Immunology, Vol. 38, No. 5, 05.2008, p. 1275-1286.

Research output: Contribution to journalArticle

Totsuka, T, Kanai, T, Nemoto, Y, Tomita, T, Tsuchiya, K, Sakamoto, N, Okamoto, R & Watanabe, M 2008, 'Immunosenescent colitogenic CD4+ T cells convert to regulatory cells and suppress colitis', European Journal of Immunology, vol. 38, no. 5, pp. 1275-1286. https://doi.org/10.1002/eji.200737914
Totsuka, Teruji ; Kanai, Takanori ; Nemoto, Yasuhiro ; Tomita, Takayuki ; Tsuchiya, Kiichiro ; Sakamoto, Naoya ; Okamoto, Ryuichi ; Watanabe, Mamoru. / Immunosenescent colitogenic CD4+ T cells convert to regulatory cells and suppress colitis. In: European Journal of Immunology. 2008 ; Vol. 38, No. 5. pp. 1275-1286.
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