Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers

Yuichi Uchino, Miki Uchino, Norihiko Yokoi, Murat Dogru, Motoko Kawashima, Aoi Komuro, Yukiko Sonomura, Hiroaki Kato, Pablo Argüeso, Shigeru Kinoshita, Kazuo Tsubota

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Abstract

The first aim of this study was to clarify whether cigarette smoking affects tear secretion, goblet cell density, and tear MUC5AC concentration. The second purpose was to evaluate the correlations of conjunctival goblet cell density with tear MUC5AC concentration and other ocular surface evaluation factors. This cross-sectional study included 88 office workers. All subjects were required to fill in dry eye and smoking questionnaires, in addition to ocular surface evaluation. Tear wash fluid was collected from inferior fornix, and conjunctival epithelium was obtained by impression cytology. Tear MUC5AC concentration was quantified using enzyme-linked immunoassay, and conjunctival goblet cell density was counted after Periodic-acid Schiff staining. Tear MUC5AC concentration had significant positive correlation with conjunctival goblet cell density (r = 0.181, P = 0.03). In current smokers, Schirmer I test value, goblet cell density and tear MUC5AC concentration were significantly lower than non-smokers. Pack-years of smoking have significant negative correlation with goblet cell density (r = -.174, P = 0.036) and tear MUC5AC concentration (r = -.183, P = 0.028). We concluded that smoking might decrease tear secretion, goblet cell density and tear MUC5AC concentration. In addition, MUC5AC concentration in tears depends on goblet cell density in the conjunctiva among office workers.

Original languageEnglish
Article number27699
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Jun 14

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Goblet Cells
Tears
Smoking
Cell Count
Periodic Acid
Conjunctiva
Immunoenzyme Techniques
Cell Biology
Epithelium
Cross-Sectional Studies
Staining and Labeling

ASJC Scopus subject areas

  • General

Cite this

Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers. / Uchino, Yuichi; Uchino, Miki; Yokoi, Norihiko; Dogru, Murat; Kawashima, Motoko; Komuro, Aoi; Sonomura, Yukiko; Kato, Hiroaki; Argüeso, Pablo; Kinoshita, Shigeru; Tsubota, Kazuo.

In: Scientific Reports, Vol. 6, 27699, 14.06.2016.

Research output: Contribution to journalArticle

Uchino, Yuichi ; Uchino, Miki ; Yokoi, Norihiko ; Dogru, Murat ; Kawashima, Motoko ; Komuro, Aoi ; Sonomura, Yukiko ; Kato, Hiroaki ; Argüeso, Pablo ; Kinoshita, Shigeru ; Tsubota, Kazuo. / Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers. In: Scientific Reports. 2016 ; Vol. 6.
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