Impaired bone fracture healing in matrix metalloproteinase-13 deficient mice

Naoto Kosaki, Hironari Takaishi, Satoru Kamekura, Tokuhiro Kimura, Yasunori Okada, Li Minqi, Norio Amizuka, Ung il Chung, Kozo Nakamura, Hiroshi Kawaguchi, Yoshiaki Toyama, Jeanine D'Armiento

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81 Citations (Scopus)

Abstract

Vascular and cellular invasion into the cartilage is a critical step in the fracture healing. Matrix metalloproteinase-13 (MMP-13) is a member of the zinc-dependent endopeptidase family and plays an important role in remodeling of extracellular matrix. Therefore we investigated the possible involvement of MMP-13 in a murine model of stabilized bone fracture healing. Repair of the fracture in MMP-13 deficient (MMP-13-/-) mice was significantly delayed and characterized by a retarded cartilage resorption in the fracture callus. Immunohistochemistry indicated severe defects in vascular penetration and chondroclast recruitment to the fracture callus in MMP-13-/- mice. Consistent with the observations, the chondrocyte pellets cultured from the MMP13-/- mice exhibited diminished angiogenic activities when the pellets were co-cultured with endothelial cells. These results suggest that MMP-13 is crucial to the process of angiogenesis during healing of fracture, especially in the cartilage resorption process.

Original languageEnglish
Pages (from-to)846-851
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume354
Issue number4
DOIs
Publication statusPublished - 2007 Mar 23

Keywords

  • Angiogenesis
  • Chondroclast
  • Extracellular matrix
  • Fracture healing
  • MMP-13

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Kosaki, N., Takaishi, H., Kamekura, S., Kimura, T., Okada, Y., Minqi, L., Amizuka, N., Chung, U. I., Nakamura, K., Kawaguchi, H., Toyama, Y., & D'Armiento, J. (2007). Impaired bone fracture healing in matrix metalloproteinase-13 deficient mice. Biochemical and Biophysical Research Communications, 354(4), 846-851. https://doi.org/10.1016/j.bbrc.2006.12.234