Impairment of cell adhesion by expression of the mutant neurofibromatosis type 2 (NF2) genes which lack exons in the ERM-homology domain

Hisashi Koga, Norie Araki, Hideo Takeshima, Toru Nishi, Toru Hirota, Yoriyoshi Kimura, Mitsuyoshi Nakao, Hideyuki Saya

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Neurofibromatosis 2 (NF2) is an inherited disorder characterized by a predisposition to multiple intracranial tumors. The protein encoded by the NF2 gene has striking similarities to ezrin, radixin and moesin (ERM) proteins which link membrane proteins to the cytoskeleton. Therefore, it can be speculated that the disruption of cytoskeletal organization by alterations in the NF2 gene is involved in the development of tumors. It has been reported that the majority of NF2 mutations were nonsense or frameshift mutations that result in premature termination of translation. To facilitate the detection of these mutations, we performed protein truncation test and found that 11 of 14 NF2 patients had truncational mutations (79%). Seven of the 11 patients (64%) had a splicing abnormality which lead to absence of exons in the ERM homology domain. To examine the biological significance of the exon-missing mutations in the ERM homology domain, we expressed the wild-type (wt-NF2) and the various mutant NF2s (mu-NF2s) in a fibroblast cell line by using both liposome-mediated transfection and nuclear microinjection of the expression plasmids. The wt-NF2 showed intense punctate staining in the perinuclear cytoplasm in addition to overall staining of the submembranous area, whereas the mu-NF2s lacking exons in the ERM homology domain showed granular staining at the perinuclear region without any accumulation at the submembrane region. Microinjection of wt-NF2 cDNA into the nucleus of VA13 cells revealed that wt-NF2 protein induced a progressive elongation of cell processes. Furthermore, cells that expressed mu-NF2 had decreased adhesion, which resulted in detachment from the substratum. These findings suggested that the exon-missing mutations in the ERM-homology domain may affect cell membrane-cytoskeleton signaling and consequently disrupt cell-to-cell or cell-to-matrix interaction.

Original languageEnglish
Pages (from-to)801-810
Number of pages10
JournalOncogene
Volume17
Issue number7
Publication statusPublished - 1998 Aug 20
Externally publishedYes

Fingerprint

Neurofibromatosis 2 Genes
Neurofibromatosis 2
Cell Adhesion
Exons
Mutation
Microinjections
Staining and Labeling
Cytoskeleton
Neurofibromin 2
Frameshift Mutation
Proteins
ezrin
radixin
moesin
Nonsense Codon
Cell Nucleus
Liposomes
Transfection
Neoplasms
Membrane Proteins

Keywords

  • Exon-missing
  • MBPs binding domain
  • NF2 gene
  • Nuclear microinjection
  • Protein truncation test (PTT)

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Impairment of cell adhesion by expression of the mutant neurofibromatosis type 2 (NF2) genes which lack exons in the ERM-homology domain. / Koga, Hisashi; Araki, Norie; Takeshima, Hideo; Nishi, Toru; Hirota, Toru; Kimura, Yoriyoshi; Nakao, Mitsuyoshi; Saya, Hideyuki.

In: Oncogene, Vol. 17, No. 7, 20.08.1998, p. 801-810.

Research output: Contribution to journalArticle

Koga, H, Araki, N, Takeshima, H, Nishi, T, Hirota, T, Kimura, Y, Nakao, M & Saya, H 1998, 'Impairment of cell adhesion by expression of the mutant neurofibromatosis type 2 (NF2) genes which lack exons in the ERM-homology domain', Oncogene, vol. 17, no. 7, pp. 801-810.
Koga, Hisashi ; Araki, Norie ; Takeshima, Hideo ; Nishi, Toru ; Hirota, Toru ; Kimura, Yoriyoshi ; Nakao, Mitsuyoshi ; Saya, Hideyuki. / Impairment of cell adhesion by expression of the mutant neurofibromatosis type 2 (NF2) genes which lack exons in the ERM-homology domain. In: Oncogene. 1998 ; Vol. 17, No. 7. pp. 801-810.
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