Impairment of decidualization in SRC-deficient mice

Aki Shimizu, Tetsuo Maruyama, Kayoko Tamaki, Hiroshi Uchida, Hironori Asada, Yasunori Yoshimura

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Many signaling events induced by ovarian steroid hormones, cytokines, and growth factors are involved in the process of decidualization of human and rodent endometrium. We have reported previously that tyrosine kinase activation of SRC functionally participates in decidualization of human endometrial stromal cells. To address its essential role in decidualization, we examined, using wild-type and Src knockout mice, whether the process of decidualization was impaired in the absence of SRC. Immunohistochemistry using an antibody specific for the active form of SRC revealed that the active SRC was expressed prominently in the decidualizing stromal cells of the pregnant wild-type mouse. Moreover, the active SRC was upregulated in the uterine horn with artificially stimulated decidual reaction. In comparison with wild-type and Src heterozygous mice, the uterus of Src null mice showed no apparent decidual response following artificial stimulation. Ovarian steroid-induced decidualization in vitro, as determined by morphological changes and expression of decidual/trophoblast prolactin-related protein and prostaglandin-endoperoxide synthase 2 (also known as Cox2), both of which are decidualization markers, did not occur in a timely fashion in endometrial stromal cells isolated from the uteri of SRC-deficient mice compared to those from wild-type and Src heterozygous mice. Our results collectively suggest that SRC is an indispensable signaling component for maximal decidualization in mice.

Original languageEnglish
Pages (from-to)1219-1227
Number of pages9
JournalBiology of reproduction
Volume73
Issue number6
DOIs
Publication statusPublished - 2005 Dec 1

Keywords

  • Decidua
  • Estradiol
  • Female reproductive tract
  • Kinases
  • Progesterone

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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