Implication of "Down syndrome cell adhesion molecule" in the hippocampal neurogenesis of ischemic monkeys

Tetsumori Yamashima, Boryana K. Popivanova, Jianzhang Guo, Susumu Kotani, Tomohiko Wakayama, Shoichi Iseki, Kazunobu Sawamoto, Hideyuki Okano, Chifumi Fujii, Naofumi Mukaida, Anton B. Tonchev

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8 Citations (Scopus)

Abstract

Molecular signals regulating adult neurogenesis in primates are largely unknown. Here the authors used differential display to analyze gene expression changes that occur in dentate gyrus of adult monkeys after transient global cerebral ischemia. Among 14 genes upregulated, the authors focused on Down syndrome cell adhesion molecule (DSCAM) known to play crucial role during neuronal development, and characterized its expression pattern at the protein level. In contrast with approximately threefold upregulation of Dscam gene on days 5 and 7, immunoblotting and immunofluorescence analyses using specific antibodies showed a gradual decrease of DSCAM after ischemia until day 9 followed by recovery on day 15. In the control, immunofluorescence reactivity of DSCAM was detected in dentate gyrus granule cells and CA4 neurons but decreased after ischemia, being compatible with the immunoblotting data. However, in the subgranular zone, cerebral ischemia led to a marked increase of DSCAM-positive cells on days 9 and 15. DSCAM upregulation was seen in two cell types: one is immature neurons positive for polysialylated neural cell adhesion molecule or βIII-tubulin, while another is astrocytes positive for S100β. Young astrocytes were in intimate contact with newly generated neurons in the subgranular zone. These data suggest implication of DSCAM in the adult neurogenesis of primate hippocampus upregulated after ischemia.

Original languageEnglish
Pages (from-to)924-935
Number of pages12
JournalHippocampus
Volume16
Issue number11
DOIs
Publication statusPublished - 2006

Keywords

  • Cerebral ischemia
  • Down syndrome
  • Neural progenitor
  • Primate
  • Subgranular zone

ASJC Scopus subject areas

  • Cognitive Neuroscience

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    Yamashima, T., Popivanova, B. K., Guo, J., Kotani, S., Wakayama, T., Iseki, S., Sawamoto, K., Okano, H., Fujii, C., Mukaida, N., & Tonchev, A. B. (2006). Implication of "Down syndrome cell adhesion molecule" in the hippocampal neurogenesis of ischemic monkeys. Hippocampus, 16(11), 924-935. https://doi.org/10.1002/hipo.20223