Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis

Shinzo Kato, H. Ishii, S. Kano, S. Hagihara, T. Todoroki, S. Nagata, H. Takahashi, M. Nagasaka, J. Sato, M. Tsuchiya

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We describe an improved method for determination of alcohol dehydrogenase (EC 1.1.1.1) activity in 60 μL of human serum, based on conversion of ethanol to acetaldehyde with simultaneous reduction of NAD+ in glycine NaOH buffer (pH 9.0) at 37°C in a centrifugal analyzer. The final concentration of NAD+ was 10 mmol/L and ethanol was 20 mmol/L. The dilution curve was linear with enzyme activity up to 200 U/L, and results by this method correlated well with those by a manual method (N Engl J Med 279: 241-248, 1968). Within-run precision (CV) was 0.9 to 8.2% over the range of 4.5 to 88.1 U/L, and day-to-day precision was 5.4 to 5.6%. In sera from 198 healthy individuals, mean alcohol dehydrogenase activity was 1.6 (SD 1.2, range 0-5) U/L. To evaluate the clinical utility of determining alcohol dehydrogenase, we measured the activity of alanine aminotransferase and alcohol dehydrogenase in sera from 470 patients with various diseases in our hospital, and found that results for the two enzymes did not correlate well.

Original languageEnglish
Pages (from-to)1817-1820
Number of pages4
JournalClinical Chemistry
Volume30
Issue number11
Publication statusPublished - 1984

Fingerprint

Alcohol Dehydrogenase
Assays
Serum
NAD
Alanine Dehydrogenase
Ethanol
Acetaldehyde
Enzyme activity
Enzymes
Alanine Transaminase
Glycine
Dilution
Buffers

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Kato, S., Ishii, H., Kano, S., Hagihara, S., Todoroki, T., Nagata, S., ... Tsuchiya, M. (1984). Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis. Clinical Chemistry, 30(11), 1817-1820.

Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis. / Kato, Shinzo; Ishii, H.; Kano, S.; Hagihara, S.; Todoroki, T.; Nagata, S.; Takahashi, H.; Nagasaka, M.; Sato, J.; Tsuchiya, M.

In: Clinical Chemistry, Vol. 30, No. 11, 1984, p. 1817-1820.

Research output: Contribution to journalArticle

Kato, S, Ishii, H, Kano, S, Hagihara, S, Todoroki, T, Nagata, S, Takahashi, H, Nagasaka, M, Sato, J & Tsuchiya, M 1984, 'Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis', Clinical Chemistry, vol. 30, no. 11, pp. 1817-1820.
Kato S, Ishii H, Kano S, Hagihara S, Todoroki T, Nagata S et al. Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis. Clinical Chemistry. 1984;30(11):1817-1820.
Kato, Shinzo ; Ishii, H. ; Kano, S. ; Hagihara, S. ; Todoroki, T. ; Nagata, S. ; Takahashi, H. ; Nagasaka, M. ; Sato, J. ; Tsuchiya, M. / Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis. In: Clinical Chemistry. 1984 ; Vol. 30, No. 11. pp. 1817-1820.
@article{fb2b95d9b7854b3dae79457cf5ae07c5,
title = "Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis",
abstract = "We describe an improved method for determination of alcohol dehydrogenase (EC 1.1.1.1) activity in 60 μL of human serum, based on conversion of ethanol to acetaldehyde with simultaneous reduction of NAD+ in glycine NaOH buffer (pH 9.0) at 37°C in a centrifugal analyzer. The final concentration of NAD+ was 10 mmol/L and ethanol was 20 mmol/L. The dilution curve was linear with enzyme activity up to 200 U/L, and results by this method correlated well with those by a manual method (N Engl J Med 279: 241-248, 1968). Within-run precision (CV) was 0.9 to 8.2{\%} over the range of 4.5 to 88.1 U/L, and day-to-day precision was 5.4 to 5.6{\%}. In sera from 198 healthy individuals, mean alcohol dehydrogenase activity was 1.6 (SD 1.2, range 0-5) U/L. To evaluate the clinical utility of determining alcohol dehydrogenase, we measured the activity of alanine aminotransferase and alcohol dehydrogenase in sera from 470 patients with various diseases in our hospital, and found that results for the two enzymes did not correlate well.",
author = "Shinzo Kato and H. Ishii and S. Kano and S. Hagihara and T. Todoroki and S. Nagata and H. Takahashi and M. Nagasaka and J. Sato and M. Tsuchiya",
year = "1984",
language = "English",
volume = "30",
pages = "1817--1820",
journal = "Clinical Chemistry",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "11",

}

TY - JOUR

T1 - Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis

AU - Kato, Shinzo

AU - Ishii, H.

AU - Kano, S.

AU - Hagihara, S.

AU - Todoroki, T.

AU - Nagata, S.

AU - Takahashi, H.

AU - Nagasaka, M.

AU - Sato, J.

AU - Tsuchiya, M.

PY - 1984

Y1 - 1984

N2 - We describe an improved method for determination of alcohol dehydrogenase (EC 1.1.1.1) activity in 60 μL of human serum, based on conversion of ethanol to acetaldehyde with simultaneous reduction of NAD+ in glycine NaOH buffer (pH 9.0) at 37°C in a centrifugal analyzer. The final concentration of NAD+ was 10 mmol/L and ethanol was 20 mmol/L. The dilution curve was linear with enzyme activity up to 200 U/L, and results by this method correlated well with those by a manual method (N Engl J Med 279: 241-248, 1968). Within-run precision (CV) was 0.9 to 8.2% over the range of 4.5 to 88.1 U/L, and day-to-day precision was 5.4 to 5.6%. In sera from 198 healthy individuals, mean alcohol dehydrogenase activity was 1.6 (SD 1.2, range 0-5) U/L. To evaluate the clinical utility of determining alcohol dehydrogenase, we measured the activity of alanine aminotransferase and alcohol dehydrogenase in sera from 470 patients with various diseases in our hospital, and found that results for the two enzymes did not correlate well.

AB - We describe an improved method for determination of alcohol dehydrogenase (EC 1.1.1.1) activity in 60 μL of human serum, based on conversion of ethanol to acetaldehyde with simultaneous reduction of NAD+ in glycine NaOH buffer (pH 9.0) at 37°C in a centrifugal analyzer. The final concentration of NAD+ was 10 mmol/L and ethanol was 20 mmol/L. The dilution curve was linear with enzyme activity up to 200 U/L, and results by this method correlated well with those by a manual method (N Engl J Med 279: 241-248, 1968). Within-run precision (CV) was 0.9 to 8.2% over the range of 4.5 to 88.1 U/L, and day-to-day precision was 5.4 to 5.6%. In sera from 198 healthy individuals, mean alcohol dehydrogenase activity was 1.6 (SD 1.2, range 0-5) U/L. To evaluate the clinical utility of determining alcohol dehydrogenase, we measured the activity of alanine aminotransferase and alcohol dehydrogenase in sera from 470 patients with various diseases in our hospital, and found that results for the two enzymes did not correlate well.

UR - http://www.scopus.com/inward/record.url?scp=0021732087&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021732087&partnerID=8YFLogxK

M3 - Article

VL - 30

SP - 1817

EP - 1820

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 11

ER -